Expression of adropin in rat brain, cerebellum, kidneys, heart, liver, and pancreas in streptozotocin-induced diabetes

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dc.contributor.authorAydin, Suleyman
dc.contributor.authorKuloglu, Tuncay
dc.contributor.authorAydin, Suna
dc.contributor.authorEren, Mehmet Nesimi
dc.contributor.authorYilmaz, Musa
dc.contributor.authorKalayci, Mehmet
dc.contributor.authorSahin, Ibrahim
dc.date.accessioned2024-04-24T16:02:17Z
dc.date.available2024-04-24T16:02:17Z
dc.date.issued2013
dc.departmentDicle Üniversitesien_US
dc.description.abstractWe have investigated how diabetes affects the expression of adropin (ADR) in rat brain, cerebellum, kidneys, heart, liver, and pancreas tissues. The rats in the diabetic group were administered an intraperitoneal (i.p.) injection of a single dose of 60 mg/kg streptozotocin (STZ) dissolved in a 0.1 M phosphate-citrate buffer (pH 4.5). The rats were maintained in standard laboratory conditions in a temperature between 21 and 23 A degrees C and a relative humidity of 70 %, under a 12-h light/dark cycle. The animals were fed a standard commercial pellet diet. After 10 weeks, the animals were sacrified. ADR concentrations in the serum and tissue supernatants were measured by ELISA, and immunohistochemical staining was used to follow the expression of the hormones in the brain, cerebellum, kidneys, heart, liver, and pancreas tissues. The quantities were then compared. Increased ADR immunoreaction was seen in the brain, cerebellum, kidneys, heart, liver, and pancreas in the diabetes-induced rats compared to control subjects. ADR was detected in the brain (vascular area, pia mater, neuroglial cell, and neurons), cerebellum (neuroglial cells, Purkinje cells, vascular areas, and granular layer), kidneys (glomerulus, peritubular interstitial cells, and peritubular capillary endothelial cells), heart (endocardium, myocardium, and epicardium), liver (sinusoidal cells), and pancreas (serous acini). Its concentrations (based on mg/wet weight tissues) in these tissues were measured by using ELISA showed that the levels of ADR were higher in the diabetic rats compared to the control rats. Tissue ADR levels based on mg/wet weight tissues were as follows: Pancreas > liver > kidney > heart > brain > cerebellar tissues. Evidence is presented that shows ADR is expressed in various tissues in the rats and its levels increased in STZ-induced diabetes; however, this effect on the pathophysiology of the disorder remains to be understood.en_US
dc.identifier.doi10.1007/s11010-013-1660-4
dc.identifier.endpage81en_US
dc.identifier.issn0300-8177
dc.identifier.issn1573-4919
dc.identifier.issue1-2en_US
dc.identifier.pmid23620340
dc.identifier.scopus2-s2.0-84879799049
dc.identifier.scopusqualityQ1
dc.identifier.startpage73en_US
dc.identifier.urihttps://doi.org/10.1007/s11010-013-1660-4
dc.identifier.urihttps://hdl.handle.net/11468/14728
dc.identifier.volume380en_US
dc.identifier.wosWOS:000321131100008
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMolecular and Cellular Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAdropin Expressionen_US
dc.subjectImmunohistochemistryen_US
dc.subjectStz-Induced Diabetesen_US
dc.subjectRat Tissuesen_US
dc.titleExpression of adropin in rat brain, cerebellum, kidneys, heart, liver, and pancreas in streptozotocin-induced diabetesen_US
dc.titleExpression of adropin in rat brain, cerebellum, kidneys, heart, liver, and pancreas in streptozotocin-induced diabetes
dc.typeArticleen_US

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