Comparison of culture and PCR methods in the diagnosis of bacterial meningitis

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dc.authorid0000-0002-1173-5286en_US
dc.authorid0000-0003-4577-4475en_US
dc.contributor.authorBaşpınar, Emel Ödemiş
dc.contributor.authorDayan, Saim
dc.contributor.authorBekçibaşı, Muhammed
dc.contributor.authorTekin, Recep
dc.contributor.authorAyaz, Celal
dc.contributor.authorDeveci, Özcan
dc.contributor.authorHoşoğlu, Salih
dc.date.accessioned2024-03-06T13:10:58Z
dc.date.available2024-03-06T13:10:58Z
dc.date.issued2017en_US
dc.departmentDicle Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Ana Bilim Dalıen_US
dc.description.abstractOur aim in this study is to compare the standard culture method with the multiplex PCR and the Speed-Oligo® Bacterial Meningitis Test (SO-BMT) – a hybridization-based molecular test method – during the CSF examination of the patients with the pre-diagnosis of acute bacterial meningitis. For the purposes of this study, patients with acute bacterial meningitis treated at the Dicle University Medical Faculty Hospital, Infectious Diseases and Clinical Microbiology Clinic between December 2009 and April 2012 were retrospectively evaluated. The diagnosis of bacterial meningitis was made based on the clinical findings, laboratory test anomalies, CSF analysis results, and the radiological images. Growth was observed in the CSF cultures of 10 out of the 57 patients included in the study (17.5%) and Streptococcus pneumoniae was isolated in all of them. The CSF samples of 34 patients (59.6%) were positive according to the SO-BMT and S. pneumoniae was detected in 33 of the samples (97.05%), while Neisseria meningitidis was found in 1 sample (2.95%). In a total of 10 patients, S. pneumoniae was both isolated in the CSF culture and detected in the SO-BMT. The culture and the SO-BMT were negative in 23 of the CSF samples. There was no sample in which the CSF culture was positive although the SO-BMT was negative. While SO-BMT seems to be a more efficient method than bacterial culturing to determine the pathogens that most commonly cause bacterial meningitis in adults, further studies conducted on larger populations are needed in order to assess its efficiency and uses.en_US
dc.identifier.citationBaşpınar, E. Ö., Dayan, S., Bekçibaşı, M., Tekin, R., Ayaz, C., Deveci, Ö. ve diğerleri. (2017). Comparison of culture and PCR methods in the diagnosis of bacterial meningitis. Brazilian Journal of Microbiology, 48(2), 232-236.en_US
dc.identifier.doi10.1016/j.bjm.2016.06.014
dc.identifier.endpage236en_US
dc.identifier.issn1517-8382
dc.identifier.issue2en_US
dc.identifier.pmid27793541
dc.identifier.scopus2-s2.0-85016944901
dc.identifier.scopusqualityQ3
dc.identifier.startpage232en_US
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S1517838216300107?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/11468/13542
dc.identifier.volume48en_US
dc.identifier.wosWOS:000401456800011
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorDayan, Saim
dc.institutionauthorTekin, Recep
dc.institutionauthorBekçibaşı, Muhammed
dc.institutionauthorAyaz, Celal
dc.institutionauthorDeveci, Özcan
dc.institutionauthorHoşoğlu, Salih
dc.language.isoenen_US
dc.publisherElsevier Editora Ltdaen_US
dc.relation.ispartofBrazilian Journal of Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBacterial meningitisen_US
dc.subjectCultureen_US
dc.subjectPCRen_US
dc.subjectSpeed-Oligoen_US
dc.titleComparison of culture and PCR methods in the diagnosis of bacterial meningitisen_US
dc.titleComparison of culture and PCR methods in the diagnosis of bacterial meningitis
dc.typeArticleen_US

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