Comparison of morphological similarities and differences between liquid-based cytology and conventional techniques of serous effusion cytology specimens

Basit Öğe Kaydı
dc.authorid0000-0002-0645-8755en_US
dc.contributor.authorBaykuş, Nurhan
dc.contributor.authorÖzekinci, Selver
dc.contributor.authorErdem, Zeynep Betül
dc.contributor.authorVurgun, Eren
dc.contributor.authorYıldız, Fetin Rüştü
dc.date.accessioned2023-02-28T13:30:48Z
dc.date.available2023-02-28T13:30:48Z
dc.date.issued2022en_US
dc.departmentDicle Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Tıbbi Patoloji Ana Bilim Dalıen_US
dc.description.abstractIntroduction: The aim of this study is to discover a fast and efficient method for the diagnosis of serous effusion cytology specimens by comparing the cytomorphological features of SurePath (SP) smears and smears prepared by cytospin. After the macroscopic features of the incoming material were recorded, it was divided into 2 for conventional technique (CT) and liquid-based technique. Cytospin was used for CT and SurePath for liquid-based technique in this study. Materials and Methods: 243 serous effusions (33 thoracentesis and 92 paracentesis fluids, 118 peritoneal lavage fluids) were investigated. After shaking the effusion gently, it was centrifuged for 5 min at 1,250 rpm for cytospin smear. SP smear was prepared according to the "BD PrepStain slide processor". Two smears were prepared with these 2 methods and then stained with Papanicolaou. The smears were examined under a light microscope in terms of fixation, background, cellularity, nucleus, and structural features. All statistical analysis of the data was performed using the SPSS 17.0 software. For each microscopic feature, the chi(2) test was used to assess the significance of the relationship between cytospin and SP, and level of agreement in between the methods was assessed using the kappa statistic. Results: A statistically significant difference was observed between the 2 methods in background (p < 0.001), cellularity (p < 0.001), nucleus features (p < 0.001), and structural features (p < 0.05). There was no significant difference in fixation. Low level of agreement was observed with the kappa statistic in fixation, background, and cellularity. Moderate level of agreement was observed in the nucleus and structural feature groups with the kappa statistic. Discussion/Conclusion: Although there are advantages of liquid-based technique such as standardized fixation and cleaner background, since the cellular and background components required for morphological analysis and diagnosis are better preserved in cytospin, it is considered to be better to use liquid-based technique not alone but together with CT.en_US
dc.identifier.citationBaykuş, N., Özekinci, S., Erdem, Z.B., Vurgun, E. ve Yıldız, F.R. (2022). Comparison of morphological similarities and differences between liquid-based cytology and conventional techniques of serous effusion cytology specimens. Acta Cytologica, 66(2), 159-164.en_US
dc.identifier.doi10.1159/000521052
dc.identifier.endpage164en_US
dc.identifier.issn0001-5547
dc.identifier.issn1938-2650
dc.identifier.issue2en_US
dc.identifier.pmid34969029
dc.identifier.scopus2-s2.0-85123536026
dc.identifier.scopusqualityQ2
dc.identifier.startpage159en_US
dc.identifier.urihttps://www.karger.com/Article/Abstract/521052
dc.identifier.urihttps://hdl.handle.net/11468/11292
dc.identifier.volume66en_US
dc.identifier.wosWOS:000740842100001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÖzekinci, Selver
dc.language.isoenen_US
dc.publisherKargeren_US
dc.relation.ispartofActa Cytologica
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCytopreparationsen_US
dc.subjectLiquid-based cytologyen_US
dc.subjectSerous effusionsen_US
dc.titleComparison of morphological similarities and differences between liquid-based cytology and conventional techniques of serous effusion cytology specimensen_US
dc.titleComparison of morphological similarities and differences between liquid-based cytology and conventional techniques of serous effusion cytology specimens
dc.typeArticleen_US

Dosyalar

Lisans paketi
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Cerrahi Tıp Bilimleri Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu