Diagnostic accuracy of digital Brix and serum total protein refractometers in estimating different passive immunity levels in dairy calves

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dc.contributor.authorAkkose, Mehmet
dc.contributor.authorKutsal, Hueseyin Gokhan
dc.contributor.authorKurban, Mert
dc.contributor.authorcinar, Elif Merve
dc.contributor.authorPolat, Yadigar
dc.contributor.authorCengiz, Murat
dc.date.accessioned2024-04-24T16:18:36Z
dc.date.available2024-04-24T16:18:36Z
dc.date.issued2022
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe aim of this study was to determine the diagnostic accuracy of digital serum total protein (TP) and digital Brix refractometers in estimating different passive immunity levels (<10, <18, <25 mg/mL) in dairy calves. Blood samples were collected from 260 apparently healthy Holstein calves, aged 2-7 days. Serum IgG concentrations were measured using digital Brix and TP refractometers and the radial immunodiffusion (RID) assay, as the gold standard. Data were analyzed by a receiver operating characteristics (ROC) analysis, the area under the ROC curves (AUC) and Cohen's kappa (kappa). Optimal thresholds were determined as < 8.4, < 9.0 and < 9.4% for the digital Brix refractometer, and < 5.0, < 5.4 and < 5.8 g/dL for the serum TP refractometer in estimating IgG concentrations of < 10, < 18, < 25 mg/mL, respectively. The sensitivity (Se) and specificity (Sp) of the Brix refractometer were 96.3% and 88.8% for < 8.4% Brix, 97.0% and 83.4% for < 9.0% Brix, and 85.5% and 77.8% for < 9.4% Brix, respectively. The Se and Sp of the serum TP refractometer were 96.3% and 90.1% for < 5.0 g/ dL, 91.0% and 89.6% for < 5.4 g/dL, 79.6% and 85.2% for < 5.8 g/dL, respectively. The discriminant ability of the refractometers was moderately accurate in estimating IgG concentrations of < 10 and < 18 mg/mL, and highly accurate in estimating IgG concentrations of < 25 mg/mL. Both refractometers substantially agreed with RID-IgG results and almost perfectly agreed with each other. In conclusion, the digital Brix and digital serum TP refractometers offer a good utility for determining different passive immunity levels in dairy calves.en_US
dc.description.sponsorshipGeneral Directorate of Agricultural Enterprises (Tarim Isletmeleri Genel Mudurlugu, TIGEM)en_US
dc.description.sponsorshipThe authors would like to thank the technical staff of the Ceylanpinar and Dalaman Agricultural Enterprises for their assistance. The authors are grateful to Dr Ufuk Kaya from Department of Biostatistics in Faculty of Veterinary Medicine, Hatay Mustafa Kemal University for his valuable contributions in statistical analysis. This study was supported by the General Directorate of Agricultural Enterprises (Tarim Isletmeleri Genel Mudurlugu, TIGEM).en_US
dc.identifier.doi10.1016/j.vetimm.2022.110442
dc.identifier.issn0165-2427
dc.identifier.issn1873-2534
dc.identifier.pmid35662038
dc.identifier.scopus2-s2.0-85131361550
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.vetimm.2022.110442
dc.identifier.urihttps://hdl.handle.net/11468/16195
dc.identifier.volume249en_US
dc.identifier.wosWOS:000811499300001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofVeterinary Immunology and Immunopathology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBrix Refractometeren_US
dc.subjectCalfen_US
dc.subjectIggen_US
dc.subjectTransfer Of Passive Immunityen_US
dc.subjectSerum Total Proteinen_US
dc.titleDiagnostic accuracy of digital Brix and serum total protein refractometers in estimating different passive immunity levels in dairy calvesen_US
dc.titleDiagnostic accuracy of digital Brix and serum total protein refractometers in estimating different passive immunity levels in dairy calves
dc.typeArticleen_US

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