Patterns of Gene Expression in the Bovine Corpus Luteum Following Repeated Intrauterine Infusions of Low Doses of Prostaglandin F2alpha

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dc.contributor.authorAtli, Mehmet O.
dc.contributor.authorBender, Robb W.
dc.contributor.authorMehta, Vatsal
dc.contributor.authorBastos, Michele R.
dc.contributor.authorLuo, Wenxiang
dc.contributor.authorVezina, Chad M.
dc.contributor.authorWiltbank, Milo C.
dc.date.accessioned2024-04-24T17:08:08Z
dc.date.available2024-04-24T17:08:08Z
dc.date.issued2012
dc.departmentDicle Üniversitesien_US
dc.description.abstractNatural luteolysis involves multiple pulses of prostaglandin F2alpha (PGF) released by the nonpregnant uterus. This study investigated expression of 18 genes from five distinct pathways, following multiple low-dose pulses of PGF. Cows on Day 9 of the estrous cycle received four intrauterine infusions of 0.25 ml of phosphate-buffered saline (PBS) or PGF (0.5 mg of PGF in 0.25 ml of PBS) at 6-h intervals. A luteal biopsy sample was collected 30 min after each PBS or PGF infusion. There were four treatment groups: Control (n = 5; 4 PBS infusions), 4XPGF (4 PGF infusions; n = 5), 2XPGF-non-regressed (2 PGF infusions; n = 5; PGF-PBS-PGF-PBS; no regression after treatments), and 2XPGF-regressed (PGF-PBS-PGF-PBS; regression after treatments; n = 5). As expected, the first PGF pulse increased mRNA for the immediate early genes JUN, FOS, NR4A1, and EGR1 but unexpectedly also increased mRNA for steroidogenic (STAR) and angiogenic (VEGFA) pathways. The second PGF pulse induced immediate early genes and genes related to immune system activation (IL1B, FAS, FASLG, IL8). However, mRNA for VEGFA and STAR were decreased by the second PGF infusion. After the third and fourth PGF pulses, a distinctly luteolytic pattern of gene expression was evident, with inhibition of steroidogenic and angiogenic pathways, whereas, there was induction of pathways for immune system activation and production of PGF. The pattern of PGF-induced gene expression was similar in corpus luteum not destined for luteolysis (2X-non-regressed) after the first PGF pulse but was very distinct after the second PGF pulse. Thus, although the initial PGF pulse induced mRNA for many pathways, the second and later pulses of PGF appear to have set the distinct pattern of gene expression that result in luteolysis.en_US
dc.description.sponsorshipNational Institutes of Health [R01 HD050616]; Wisconsin Experiment Station; Council of Higher Education, Turkeyen_US
dc.description.sponsorshipSupported by National Institutes of Health grant R01 HD050616, the Wisconsin Experiment Station, and The Council of Higher Education, Turkey.en_US
dc.identifier.doi10.1095/biolreprod.111.094870
dc.identifier.issn0006-3363
dc.identifier.issue4en_US
dc.identifier.pmid22262696
dc.identifier.scopus2-s2.0-84864008561
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1095/biolreprod.111.094870
dc.identifier.urihttps://hdl.handle.net/11468/17219
dc.identifier.volume86en_US
dc.identifier.wosWOS:000306549500018
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherSoc Study Reproductionen_US
dc.relation.ispartofBiology of Reproduction
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCorpus Luteumen_US
dc.subjectGene Expressionen_US
dc.subjectHormone Actionen_US
dc.subjectLuteolysisen_US
dc.subjectMrnaen_US
dc.subjectProstaglandinsen_US
dc.titlePatterns of Gene Expression in the Bovine Corpus Luteum Following Repeated Intrauterine Infusions of Low Doses of Prostaglandin F2alphaen_US
dc.titlePatterns of Gene Expression in the Bovine Corpus Luteum Following Repeated Intrauterine Infusions of Low Doses of Prostaglandin F2alpha
dc.typeArticleen_US

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