mRNA Expression Profile of SFKs and Involvement of SFKs in the Regulation of LPS-Induced Erk1/2 Signaling in PBMCs of Active BD Patients

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dc.contributor.authorIrtegun-Kandemir, Sevgi
dc.contributor.authorIcen-Taskin, Irmak
dc.contributor.authorBozkurt, Mehtap
dc.contributor.authorKalkanli-Tas, Sevgi
dc.date.accessioned2024-04-24T17:18:35Z
dc.date.available2024-04-24T17:18:35Z
dc.date.issued2019
dc.departmentDicle Üniversitesien_US
dc.description.abstractBackground: Behcet's Disease (BD) is a multisystemic inflammatory disorder affecting large vessels, lungs joints, gastrointestinal and neurological systems. The pathogenesis of BD remains poorly understood. Identifying the key signaling pathway is crucial for a complete understanding of the pathogenesis of BD. Objective: The aim of this study was to determine mRNA expression level of Src family kinases (SFKs) members and their involvement in lipopolysaccharide (LPS)-induced mitogen-activated protein kinases (MAPKs) regulation in peripheral blood mononuclear cells (PBMCs) of active BD patients. Methods: Twenty- five active BD patients and twenty-five healthy controls were included in the study. PBMCs were isolated from total blood by density gradient centrifugation. The mRNA expression levels of SFKs members were measured by real-time quantitative PCR (RT-qPCR). The effect of SFKs activity on LPS-induced activation MAPKs (Erk1/2, p38 and JNK) was examined by Western blot. Results: The mRNA expression levels of Hck, Src, Lyn, Yes and Fyn were found to be slightly decreased in active BD patients compared to the control subjects, but a slight change in mRNA level of SFKs members did not impact on protein levels and protein activity. LPS-induced Erk1/2 phosphorylation was significantly increased in the absence of SFKs activity in active BD patients. However, inhibition of SFKs activity had no effect on LPS-induced phosphorylation of p38 and JNK in both controls and active BD patients. Conclusion: SFKs downregulate LPS-induced Erk1/2 phosphorylation in PBMCs of active BD patients.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [213S016]en_US
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) with 213S016 grant number.en_US
dc.identifier.doi10.2174/1871530319666190119101756
dc.identifier.endpage817en_US
dc.identifier.issn1871-5303
dc.identifier.issn2212-3873
dc.identifier.issue6en_US
dc.identifier.pmid30659554en_US
dc.identifier.scopus2-s2.0-85072312034en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage809en_US
dc.identifier.urihttps://doi.org/10.2174/1871530319666190119101756
dc.identifier.urihttps://hdl.handle.net/11468/18839
dc.identifier.volume19en_US
dc.identifier.wosWOS:000484371400010
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherBentham Science Publ Ltden_US
dc.relation.ispartofEndocrine Metabolic & Immune Disorders-Drug Targetsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBehcet's Diseaseen_US
dc.subjectLpsen_US
dc.subjectMitogen-Activated Protein Kinaseen_US
dc.subjectSrc Family Kinasesen_US
dc.subjectPbmcsen_US
dc.subjectTlr4en_US
dc.titlemRNA Expression Profile of SFKs and Involvement of SFKs in the Regulation of LPS-Induced Erk1/2 Signaling in PBMCs of Active BD Patientsen_US
dc.typeArticleen_US

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