Production and purification of novel thermostable alkaline protease from Anoxybacillus sp. KP1

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dc.contributor.authorBekler, Fatma Matpan
dc.contributor.authorAcer, Ömer
dc.contributor.authorGüven, Kemal
dc.date.accessioned2024-04-24T17:56:41Z
dc.date.available2024-04-24T17:56:41Z
dc.date.issued2015
dc.departmentDicle Üniversitesi, Fen Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractIn this study, an extracellular novel alkaline protease (EC 3.4.21-24, 99) from a thermophilic and aerobic strain of Anoxybacillus sp. KP1 has been studied. Maximum protease activity was obtained at 50 °C at pH 9.0 after 24 hours of incubation. Among the carbon and nitrogen sources used; the optimum protease production was with soluble starch, maltose, urea and casamino acid. The enzyme was purified by ammonium sulphate precipitation and Sephadex G-75 gel chromatography. Molecular weight of purified enzyme was determined as 106 kDa by SDS-PAGE. Purified protease was stable at 50-60 °C and at pH 9.0 for 1 h. The enzyme activity was increased in the presence of Ca2+, Cu2+, Tween 80 and Triton X-100, however the enzyme activity was inhibited in the presence of Hg2+, ethylene diamine tetra acetic acid (EDTA) and H2O2. Proteolytic activity was completely inhibited by phenyl methyl sulfonyl fluoride (PMSF). The enzyme seems to be a serine alkaline protease. In the presence of detergents, the protease was clearly stable and residual activity was between 73-82%.en_US
dc.identifier.citationBekler, F. M., Acer, Ö. ve Güven, K. (2015). Production and purification of novel thermostable alkaline protease from Anoxybacillus sp. KP1. Cellular and Molecular Biology, 61(4), 113-120.
dc.identifier.doi10.14715/cmb/2015.61.4.18
dc.identifier.endpage120en_US
dc.identifier.issn0145-5680
dc.identifier.issue4en_US
dc.identifier.pmid26429301
dc.identifier.scopus2-s2.0-84963647533
dc.identifier.scopusqualityQ4
dc.identifier.startpage113en_US
dc.identifier.urihttps://doi.org/10.14715/cmb/2015.61.4.18
dc.identifier.urihttps://hdl.handle.net/11468/23622
dc.identifier.volume61en_US
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherCellular and Molecular Biology Associationen_US
dc.relation.ispartofCellular and Molecular Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAnoxybacillusen_US
dc.subjectProductionen_US
dc.subjectProteaseen_US
dc.subjectPurificationen_US
dc.subjectThermophilesen_US
dc.titleProduction and purification of novel thermostable alkaline protease from Anoxybacillus sp. KP1en_US
dc.titleProduction and purification of novel thermostable alkaline protease from Anoxybacillus sp. KP1
dc.typeArticleen_US

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