Effects of estrous cycle stage and transport temperature of ovaries on in vitro maturation of canine oocytes

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dc.contributor.authorEvecen, Mithat
dc.contributor.authorCirit, Uemuet
dc.contributor.authorDemir, Kamber
dc.contributor.authorOzdas, Ozen Banu
dc.contributor.authorTas, Muzaffer
dc.contributor.authorBirler, Sema
dc.contributor.authorPabuccuoglu, Serhat
dc.date.accessioned2024-04-24T16:10:48Z
dc.date.available2024-04-24T16:10:48Z
dc.date.issued2010
dc.departmentDicle Üniversitesien_US
dc.description.abstractUnlike other domestic animals, in vitro maturation (IVM) of canine oocytes still has limited success. The present study investigated the effects of estrous cycle stage and transport temperature of ovaries on in vitro maturation of canine oocytes. The donor bitches were categorized into three groups based on stage of estrus cycle: follicular (proestrus or estrous), luteal (diestrus) and anestrus. One ovary of each pair collected from 39 mature bitches was transported in Phosphate Buffer Saline (PBS) at 4 degrees C while the other was transported at 37 degrees C. A total of 1138 Grade I COCs obtained from all ovaries were grouped and matured in modified synthetic oviduct fluid (mSOF) supplemented with follicle stimulating hormone (FSH), luteinizing hormone (LH), essential and non-essential amino acids at 38.5 degrees C in a humidified 5% CO2, 5% O-2, and 90% N-2 atmosphere for 72 h. The nuclear maturation rates were evaluated by aceto-orcein staining. Oocytes harvested from follicular and luteal ovaries have a significantly higher maturation rates (MI+MII) than the oocytes from anestrual ovaries in the 37 degrees C group (p<0.05). However, oocytes harvested from anestrual ovaries transported at 4 degrees C had the highest maturation (MI + MII) rate, and the difference between anestrual and luteal ovary groups was significant (p < 0.05). The oocytes from anestrual ovaries transported at 4 degrees C have significantly higher maturation rates than those transported at 37 degrees C (p < 0.0001). However, the transport temperature (37 or 4 degrees C) did not significantly affect the maturation (MI + MII) rates of oocytes harvested from the luteal (p = 0.61) and follicular (p = 0.48) stage ovaries. It can be concluded from this study that (1) both transport temperature and transport temperature x estrus cycle stage interaction effected the maturation rates, while estrus cycle stage alone did not, and (2) transporting canine ovaries at 4 degrees C can improve in vitro maturation rates in oocytes harvested from anestrous ovaries. (C) 2009 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.anireprosci.2009.03.004
dc.identifier.endpage165en_US
dc.identifier.issn0378-4320
dc.identifier.issn1873-2232
dc.identifier.issue1-2en_US
dc.identifier.pmid19375251
dc.identifier.scopus2-s2.0-70350702774
dc.identifier.scopusqualityQ1
dc.identifier.startpage160en_US
dc.identifier.urihttps://doi.org/10.1016/j.anireprosci.2009.03.004
dc.identifier.urihttps://hdl.handle.net/11468/15117
dc.identifier.volume117en_US
dc.identifier.wosWOS:000272481100023
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofAnimal Reproduction Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCanineen_US
dc.subjectOvaryen_US
dc.subjectEstrous Cycle Stageen_US
dc.subjectTransport Temperatureen_US
dc.subjectIn Vitro Maturationen_US
dc.titleEffects of estrous cycle stage and transport temperature of ovaries on in vitro maturation of canine oocytesen_US
dc.titleEffects of estrous cycle stage and transport temperature of ovaries on in vitro maturation of canine oocytes
dc.typeArticleen_US

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