Comparison of cryoprotective effects of iodixanol, trehalose and cysteamine on ram semen

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dc.contributor.authorCirit, Umut
dc.contributor.authorBagis, Haydar
dc.contributor.authorDemir, Kamber
dc.contributor.authorAgca, Cansu
dc.contributor.authorPabuccuoglu, Serhat
dc.contributor.authorVarisli, Omer
dc.contributor.authorClifford-Rathert, Charlotte
dc.date.accessioned2024-04-24T16:10:48Z
dc.date.available2024-04-24T16:10:48Z
dc.date.issued2013
dc.departmentDicle Üniversitesien_US
dc.description.abstractThis study was conducted to improve cryosurvival of electroejaculated (EE) ram semen in the presence of iodixanol (OptiPrep (TM)), trehalose or cysteamine. A tris-based extender was used to prepare 12 extenders containing OptiPrep (TM) (Op), trehalose (Tr) or cysteamine (Cy) alone, or different combinations of these compounds. Extenders were designated as follows: Tris (control), Op1.25 (1.25% Op, v/v), Op2.5 (2.5% Op, v/v), Op5 (5% Op, v/v), Tr50 (50 mM Tr), Tr100 (100 mM Tr), Cy (5 mM Cy), OpTr (2.5% Op and 100 mM Tr), OpCy (2.5% Op and 5 mM Cy), TrCy (100 mM Tr and 5 mM Cy), OpTrCy1 (2.5% Op, 100 mM Tr and 5 mM Cy) and OpTrCy2 (1.25% Op, 50 mM Tr and 2.5 mM Cy). A two-step dilution was used and glycerol was added at 5 degrees C in the second step. Diluted samples were equilibrated for 1 h, loaded in 0.25 mL straws and frozen in a programmable freezing machine. Supplementation of 5% OptiPrep (TM) significantly protected post-thaw progressive motility, membrane integrity, acrosomal integrity and morphological damages. Trehalose supplementation protected membrane integrity of ram sperm; however, it did not help post-thaw motility and morphology. Supplementation of 5 mM cysteamine had detrimental effect on cryosurvival of EE ram semen. These results demonstrate that the supplementation of iodixanol increases the cryosurvival of EE ram semen in a dose-dependent manner. (c) 2013 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipTUBITAK [107G093]en_US
dc.description.sponsorshipThis study was supported by TUBITAK; Project number: 107G093. We thank Dr. S. Mehmet Soylu for their help with semen collection.en_US
dc.identifier.doi10.1016/j.anireprosci.2013.03.010
dc.identifier.endpage44en_US
dc.identifier.issn0378-4320
dc.identifier.issn1873-2232
dc.identifier.issue1-4en_US
dc.identifier.pmid23602486
dc.identifier.scopus2-s2.0-84878108969
dc.identifier.scopusqualityQ1
dc.identifier.startpage38en_US
dc.identifier.urihttps://doi.org/10.1016/j.anireprosci.2013.03.010
dc.identifier.urihttps://hdl.handle.net/11468/15121
dc.identifier.volume139en_US
dc.identifier.wosWOS:000320493100006
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofAnimal Reproduction Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectRamen_US
dc.subjectElectro-Ejaculated Semenen_US
dc.subjectIodixanolen_US
dc.subjectTrehaloseen_US
dc.subjectCysteamineen_US
dc.titleComparison of cryoprotective effects of iodixanol, trehalose and cysteamine on ram semenen_US
dc.titleComparison of cryoprotective effects of iodixanol, trehalose and cysteamine on ram semen
dc.typeArticleen_US

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