Antityrosinase activity and LC-MS/MS analysis of optimized ultrasound-assisted condition extracts and fractions from strawberry tree ( Arbutus unedo L.)

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dc.authoridDemir, Serdar/0000-0001-6572-8818
dc.authoridKoyu, Halil/0000-0002-5491-9894
dc.authoridYILMAZ, Mustafa Abdullah/0000-0002-4090-7227
dc.contributor.authorDemir, Serdar
dc.contributor.authorKoyu, Halil
dc.contributor.authorYilmaz, Mustafa A.
dc.contributor.authorTarhan, Abbas
dc.contributor.authorOzturk, Sura Baykan
dc.date.accessioned2025-02-22T14:08:39Z
dc.date.available2025-02-22T14:08:39Z
dc.date.issued2024
dc.departmentDicle Üniversitesien_US
dc.description.abstractInvestigation of utilization possibilities of natural sources has been an important area for research. Tyrosinase inhibitory activity plays a key role in food and medicine industry. Strawberry tree ( Arbutus unedo ), a widely distributed plant among Mediterranean countries, possess fruits and leaves with rich bioactive phytochemicals, especially polyphenolic compounds. In this study, we aimed to investigate the antityrosinase activity of the fruit and leaf extracts of the plant, and to determine the phenolic compounds that contribute to the antityrosinase activity. In this regard, we evaluated the effect of solvent composition on the extraction of phenolic compounds from A. unedo and on its antityrosinase activity using a simplex centroid design approach, and used chromatographic and LC-MS/MS techniques. The leaf extracts prepared using EtOH:water (50:50) provided higher TPC (456.39 mg GAE/g extract) and acetone:EtOH:water (33:33:33) provided higher TFC (56.15 mg QE/g extract) values than of fruit extracts. LC-MS/MS analysis revealed 23 phenolic/ fl avonoid compounds in leaf extracts (L1-8), and major metabolites were detected as quercitrin, quinic acid, catechin, tannic acid, isoquercitrin, gallic acid, and ellagic acid. Among the leaf extracts, L3 (aceton:water, 50:50) exhibited 72.01% tyrosinase inhibition at 500 mu g/mL. After fractionation studies guided by antityrosinase activity, its subfraction L3-Fr2 exhibited 40.06% inhibition at 50 mu g/mL concentration (IC 50 : 146 +/- 7.75 mu g/mL), and catechin (113.19 mg/g), tannic acid (53.14 mg/g), ellagic acid (22.14 mg/g), gallic acid (10.27 mg/g), and epicatechin gallate (8.65 mg/ g) were determined as major metabolites. Its subfraction L3-Fr2-sub7 exhibited better antityrosinase activity (IC 50 : 206.23 +/- 9.87 mu g/mL), and quantitative analysis results revealed the presence of tannic acid (127.40 mg/g), gallic acid (13.96 mg/g), ellagic acid (7.66 mg/g), quercetin-3- O -glucuronide (5.06 mg/g), and quinic acid (3.2 mg/g) as major metabolites, and correlation analysis showed that ellagic acid and quinic acid were positively correlated with antityrosinase activity.en_US
dc.identifier.doi10.38212/2224-6614.3496
dc.identifier.issn1021-9498
dc.identifier.issue2en_US
dc.identifier.pmid38934692en_US
dc.identifier.scopus2-s2.0-85197136764en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.38212/2224-6614.3496
dc.identifier.urihttps://hdl.handle.net/11468/29541
dc.identifier.volume32en_US
dc.identifier.wosWOS:001246437900006
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherDigital Commons Bepressen_US
dc.relation.ispartofJournal of Food and Drug Analysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKA_WOS_20250222
dc.subjectAntityrosinaseen_US
dc.subjectArbutus unedoen_US
dc.subjectChemical compositionen_US
dc.subjectLC-MS/MSen_US
dc.subjectSimplex centroid designen_US
dc.titleAntityrosinase activity and LC-MS/MS analysis of optimized ultrasound-assisted condition extracts and fractions from strawberry tree ( Arbutus unedo L.)en_US
dc.typeArticleen_US

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