Apoptotic effect of thymoquinone on OVCAR3 cells via the P53 and CASP3 activation

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dc.authoridTuncer, Mehmet Cudi/0000-0001-7317-5467
dc.authoridOZTURK, Samil/0000-0002-9435-8139
dc.contributor.authorKaraosmanoglu, Ozge
dc.contributor.authorKamalak, Zeynep
dc.contributor.authorOzdemir, Ilhan
dc.contributor.authorTuncer, Mehmet Cudi
dc.contributor.authorOzturk, Tamil
dc.date.accessioned2025-02-22T14:08:47Z
dc.date.available2025-02-22T14:08:47Z
dc.date.issued2024
dc.departmentDicle Üniversitesien_US
dc.description.abstractPurpose: The limitations in cancer treatment and the inadequacy of classical methods have made it necessary to discover therapeutics in cancer treatment. The cytotoxicity of thymoquinone, which has quite different properties in terms of biological activities, in ovarian cancer cells, and the changes in the expression levels of apoptotic genes (p53/caspase-3 (casp-3)) were investigated. Methods: In the study, thymoquinone (5, 50, 100, 250 and 500 mu M and 24, 48, 72 hours) were applied to ovarian adenocarcinoma cancer cell line (OVCAR3), at different concentrations. Cytotoxic effect of thymoquinone on OVCAR-3 cells were analyzed by MTT method, and apoptotic and pro-apoptotic gene expression levels (p53, Casp-3) of thymoquinone in cancer cells were analyzed by quantitative real-time polymerase chain reaction. Results: Thymoquinone, whose effect has been revealed in many types of cancer, was shown to significantly reduce the viability of OVCAR3 cancer cells depending on the dose and time (p < 0.05). It was also determined that Casp-3 and p53 gene expressions increased in OVCAR3 cells. Conclusion: Considering the in-vitro cytotoxic activity and apoptotic gene expressions of thymoquinone, an important treatment agent, since it is a promising agent for the future of cancer treatment, more comprehensive studies may pave the way for its clinical use.en_US
dc.identifier.doi10.1590/acb399224
dc.identifier.issn0102-8650
dc.identifier.issn1678-2674
dc.identifier.pmid39536185en_US
dc.identifier.scopus2-s2.0-85209391137en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1590/acb399224
dc.identifier.urihttps://hdl.handle.net/11468/29635
dc.identifier.volume39en_US
dc.identifier.wosWOS:001356662900001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherActa Cirurgica Brasileiraen_US
dc.relation.ispartofActa Cirurgica Brasileiraen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKA_WOS_20250222
dc.subjectCell Cycle. Apoptosisen_US
dc.subjectAntineoplastic Agentsen_US
dc.subjectOvarian Neoplasmsen_US
dc.subjectCell Lineen_US
dc.titleApoptotic effect of thymoquinone on OVCAR3 cells via the P53 and CASP3 activationen_US
dc.typeArticleen_US

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