Investigation of the Effects of Thymoquinone and Dental Pulp-Derived Mesenchymal Stem Cells on Tibial Bone Defect Models

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dc.contributor.authorOzden, Ersin
dc.contributor.authorKaya, Beyza
dc.contributor.authorGuler, Ridvan
dc.date.accessioned2025-02-22T14:08:53Z
dc.date.available2025-02-22T14:08:53Z
dc.date.issued2024
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe thymoquinone obtained from Nigella sativa increases osteoblastic activity and significantly reduces the number of osteoclasts, thereby accelerating bone healing. In addition, mesenchymal stem cells isolated from various tissues are considered a potential cell source for bone regenerative therapies. The aim of this study is to investigate the effectiveness of thymoquinone, a current and novel agent, in combination with mesenchymal stem cells derived from the dental pulp in promoting bone healing. In the study, 28 male Sprague Dawley rats were used. The rats were divided into 4 groups, each consisting of 7 rats: the control group (group 1) (n=7), thymoquinone group (group 2) (n=7), stem cell group (group 3) (n=7), stem cell+thymoquinone group (group 4) (n=7). A bone defect of 4 mm in diameter and 5 mm in length was created in the left tibial bones of all rats with a trephine bur. In group 1, no procedure was applied to the defect area. Group 2 was applied thymoquinone (10 mg/kg) with oral gavage. In group 3, stem cells were used locally to the defect area. In group 4, stem cells and thymoquinone (10 mg/kg) was applied to the defect area. All rats were killed on the 28th day of the experiment. Tibia tissues extracted during sacrifice were histomorphologically examined in a fixative solution. Significant differences were found in terms of new bone formation and osteoblastic activity values in the thymoquinone (P<0.05), stem cell (P<0.05), and stem cell+thymoquinone (P<0.05) groups compared to the control group. In addition, while there was no significant difference in the thymoquinone group compared to these stem cell+thymoquinone group in terms of osteoblastic activity (P>0.05), the difference in terms of new bone formation was found to be significantly lower. No significant differences among the other groups were observed in new bone formation and osteoblastic activity (P>0.05). According to the results of our study, stem cell+thymoquinone treatment for bone defects is not only more effective than thymoquinone or stem cell treatment alone but also induces greater development of bone trabeculae, contributes to the matrix and connective tissue formation, and increases the number of osteoblasts and osteocytes involved in bone formation.en_US
dc.description.sponsorshipDicle University Scientific Research Projects Coordination Office [DIdot;S.20.012]en_US
dc.description.sponsorshipThis study is supported by Dicle University Scientific Research Projects Coordination Office with project number D & Idot;S.20.012.en_US
dc.identifier.doi10.1097/SCS.0000000000010323
dc.identifier.endpage1963en_US
dc.identifier.issn1049-2275
dc.identifier.issn1536-3732
dc.identifier.issue7en_US
dc.identifier.pmid38758543en_US
dc.identifier.scopus2-s2.0-85206115956en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1958en_US
dc.identifier.urihttps://doi.org/10.1097/SCS.0000000000010323
dc.identifier.urihttps://hdl.handle.net/11468/29695
dc.identifier.volume35en_US
dc.identifier.wosWOS:001328281900065
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherLippincott Williams & Wilkinsen_US
dc.relation.ispartofJournal of Craniofacial Surgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKA_WOS_20250222
dc.subjectBone defecten_US
dc.subjectexperimental studyen_US
dc.subjectmesenchymal stem cellen_US
dc.subjectNigella sativaen_US
dc.subjectraten_US
dc.subjectthymoquinoneen_US
dc.titleInvestigation of the Effects of Thymoquinone and Dental Pulp-Derived Mesenchymal Stem Cells on Tibial Bone Defect Modelsen_US
dc.typeArticleen_US

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