Interaction of L-Arginine-methyl ester and Sonic hedgehog in liver ischemia-reperfusion injury in the rats

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dc.contributor.authorTuncer, Mehmet Cudi
dc.contributor.authorOzturk, Hayrettin
dc.contributor.authorBuyukbayram, Huseyin
dc.contributor.authorOzturk, Hulya
dc.date.accessioned2024-04-24T17:37:33Z
dc.date.available2024-04-24T17:37:33Z
dc.date.issued2007
dc.departmentDicle Üniversitesien_US
dc.description.abstractAIM: To investigate the role of Sonic hedgehog (Shh) on the course of liver ischemia and reperfusion (I/R) in rats, and the interaction between treatment with nitric oxide donor L-Arginine-methyl ester (L-Arg) and up-regulation of Shh expression. METHODS: A total of 30 male Sprague-Dawley rats weighing 220-240 g were used in this study. Shamcontrol group (G1, n = 10): a sham operation was performed (except for liver I/R). I/R-untreated group (G2, n = 10): rats underwent liver ischemia for 1 h followed by reperfusion for 45 min. I/R-L-Arg group (G3, n = 10): after performing the same surgical procedure as in group 2, animals were treated with L-Arg. Liver tissues were taken for determination of malondialdehyde (MDA) levels, and biochemical and histological evaluations were made. RESULTS: Plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and gamma-glutamyltranspeptidase (GGT) activities were higher in group 2 than in group 3. MDA values and the hepatic injury score decreased in the L-Arg treated group compared to the I/R-untreated group. In group 2, the hepatocytes were swollen with marked vacuolization. Group 3 rats showed well-preserved liver parenchyma, with hepatocytes extending from the central vein. The morphology of the hepatocytes and the sinusoidal structures was normal, without any signs of congestion. Mild Shh positive immunostaining was detected in group 2 animals. The expression of immunoreactive cells was increased markedly in liver tissue from I/R-L-Arg rats. CONCLUSION: Our findings suggest that Shh molecules are critical factors in the pathophysiology of inflammatory liver injury induced by I/R. In addition, NO plays an important role in the immunohistochemical expression of these molecules. (c) 2007 WJG. All rights reserved.en_US
dc.identifier.endpage3846en_US
dc.identifier.issn1007-9327
dc.identifier.issn2219-2840
dc.identifier.issue28en_US
dc.identifier.pmid17657839
dc.identifier.scopus2-s2.0-34547991502
dc.identifier.scopusqualityQ1
dc.identifier.startpage3841en_US
dc.identifier.urihttps://hdl.handle.net/11468/21019
dc.identifier.volume13en_US
dc.identifier.wosWOS:000248382500010
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherBaishideng Publishing Group Incen_US
dc.relation.ispartofWorld Journal of Gastroenterology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectIschemia/Reperfusionen_US
dc.subjectLiveren_US
dc.subjectSonic Hedgehogen_US
dc.subjectL-Arginine-Methyl Esteren_US
dc.titleInteraction of L-Arginine-methyl ester and Sonic hedgehog in liver ischemia-reperfusion injury in the ratsen_US
dc.titleInteraction of L-Arginine-methyl ester and Sonic hedgehog in liver ischemia-reperfusion injury in the rats
dc.typeArticleen_US

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