Rating of putative housekeeping genes for quantitative gene expression analysis in cyclic and early pregnant equine endometrium

Basit Öğe Kaydı
dc.contributor.authorKayis, Seyit A.
dc.contributor.authorAtli, Mehmet O.
dc.contributor.authorKurar, Ercan
dc.contributor.authorBozkaya, Faruk
dc.contributor.authorSemacan, Ahmet
dc.contributor.authorAslan, Selim
dc.contributor.authorGuzeloglu, Aydin
dc.date.accessioned2024-04-24T16:10:48Z
dc.date.available2024-04-24T16:10:48Z
dc.date.issued2011
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe aim was an evaluation of a set of housekeeping genes (HKGs) to be used in the normalization of gene expression in the equine endometrium. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), hypoxanthine ribosyl transferase 1 (HPRT1), ubiquitin B (UBB), tubulin alpha 1 (TUBA1), ribosomal protein L32 (RPL32), beta-2-microglobulin (B2M), 18S rRNA (18S), and 285 rRNA (28S) HKGs were evaluated using real-time PCR and were compared in different physiological stages of the endometrium. Endometrial biopsies were obtained from mares on day of ovulation (d0, n = 4), at late diestrus (LD, n = 4), after luteolyis (AL, n = 4) of the cycle and on days 14 (P14; n = 3), 18 (P18, n = 3) and 22 (P22; n = 3) of pregnancy. A model based on REML with support of descriptive statistics was proposed in accordance with experimental design and was further confirmed with principal component analysis (PCA). Results were compared with widely used software including geNorm, BestKeeper, and NormFinder. Results indicated that GAPDH was the most stable HKG and RPL32 was ranked as the second best. 18S and 285 were found to be the least stable. The proposed model, PCA, geNorm, and BestKeeper were in agreement in detecting the most stable and the least stable HKGs in the equine endometrium during the estrous cycle and early pregnancy. (C) 2011 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipTUBITAK [TOVAG 107O035]en_US
dc.description.sponsorshipThis study was financially supported by TUBITAK grant (TOVAG 107O035 to AG). The authors would like to thank Prof. Dr. Sefa Celik for his help in real-time analysis.en_US
dc.identifier.doi10.1016/j.anireprosci.2011.02.019
dc.identifier.endpage132en_US
dc.identifier.issn0378-4320
dc.identifier.issue1-4en_US
dc.identifier.pmid21411251
dc.identifier.scopus2-s2.0-79958207830
dc.identifier.scopusqualityQ1
dc.identifier.startpage124en_US
dc.identifier.urihttps://doi.org/10.1016/j.anireprosci.2011.02.019
dc.identifier.urihttps://hdl.handle.net/11468/15119
dc.identifier.volume125en_US
dc.identifier.wosWOS:000292668800017
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofAnimal Reproduction Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHousekeeping Geneen_US
dc.subjectMixed Modelen_US
dc.subjectGenormen_US
dc.subjectNormfinderen_US
dc.subjectBestkeeperen_US
dc.subjectEquine Endometriumen_US
dc.titleRating of putative housekeeping genes for quantitative gene expression analysis in cyclic and early pregnant equine endometriumen_US
dc.titleRating of putative housekeeping genes for quantitative gene expression analysis in cyclic and early pregnant equine endometrium
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu