Localization of Estrogen Receptor Alpha and Progesterone Receptor B in the Bovine Ovary During the Follicular and Luteal Phase of the Sexual Cycle
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Tarih
2011
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Kafkas Univ, Veteriner Fakultesi Dergisi
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Ovarian steroid hormones, mainly estrogen and progesterone, play a central role in the regulation of ovarian functions. The biological effects of progesterone (PR) and estrogen (ER) are dependent on the activation of both PR and ER isoforms. In the present study, we studied the cellular distribution and localization of progesterone receptor B (PR-B) and estrogen receptor alpha (ER alpha) in the bovine ovaries during the follicular and luteal phases of the sexual cycle using immunohistochemical methods. The estrous cycle stage of 23 Holstein bovines was evaluated by gross and histological appearance of ovaries and blood steroid hormone values. Tissue samples from ovaries were fixed in 10% formaldehyde for routine histological processing. ERa immunoreactivity was observed in nuclei of granulosa cells of growing follicles at all stages from primary to mature follicles, epithelial cells of the germinal epithelium, stroma cells, theca cells, corpora lutea cells and cells of the corpora albicantia. Nuclear staining for PR-B was not detected in the granulosa cells in all sizes of follicles, but was positively stained in germinal epithelium cells, stroma cells, corpora albicantia cells and corpora lutea cells. In conclusion, ER alpha and PR-B immunoreactivity in bovine ovary were not indicate too much difference between the phases of the sexual cycle but have important differences in cell-spesific localization.
Açıklama
Anahtar Kelimeler
Bovine Ovary, Estrogen Receptor, Progesterone Receptor, Immunohistochemistry
Kaynak
Kafkas Universitesi Veteriner Fakultesi Dergisi
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
17
Sayı
5
