Intravenous Repeated Application of Low Dose ?-Galatosylceramide Boosts IFN-? Production in BALB/c Mice

Basit Öğe Kaydı
dc.contributor.authorKaya, Savas
dc.date.accessioned2024-04-24T17:24:47Z
dc.date.available2024-04-24T17:24:47Z
dc.date.issued2012
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe aim of this study is to illustrate the relationship between Alpha Galatosylceramide (alpha-GalCer) dose and alpha-GalCer induced anergy of immune system in vivo. alpha-GalCer, a glycolipid and a widely studied immune modulator extracted from a marine sponge, shows its effect in vivo via presentation on CD1d molecule, MHC I like molecule bound glycolipid antigens, to NKT cells, a lymphocyte has both TCR and NK1.1, marker of T cells and NK cells respectively. The contradictory reports about alpha-GalCer are focused on two opposite axes of immune system: cancer and autoimmunity, failure and exaggeration of Th1 type immune response, respectively. In this study, the relation between the alpha-GalCer dose and the anergy was considered to clarify this dilemma. The groups of BALB/c mice were injected with various titer of alpha-GalCer intravenous way repeatedly and serum level of IFN-gamma was measured with mouse IFN-gamma ELISA. The Dramatic difference was found between groups after 2nd application of alpha-GalCer: Low dose alpha-GalCer boosted IFN-gamma; however, high dose, usual dose cited in literature, failed to induce IFN-gamma production. Besides, low concentrations of _-GalCer succeeded considerable amount of IFN-gamma in the serum after priming. This results show that minute level of alpha-GalCer can be recognized by NKT cells, and repeated in vivo application of low dose alpha-GalCer can improve Th1 capabilities of the immune system.en_US
dc.identifier.doi10.4999/uhod.10141
dc.identifier.endpage41en_US
dc.identifier.issn1306-133X
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84859466999
dc.identifier.scopusqualityQ4
dc.identifier.startpage36en_US
dc.identifier.urihttps://doi.org/10.4999/uhod.10141
dc.identifier.urihttps://hdl.handle.net/11468/19832
dc.identifier.volume22en_US
dc.identifier.wosWOS:000302826900006
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherAkad Doktorlar Yayinevien_US
dc.relation.ispartofUhod-Uluslararasi Hematoloji-Onkoloji Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectNkt Cellsen_US
dc.subjectGalactosylceramideen_US
dc.subjectAnergyen_US
dc.subjectTh1en_US
dc.titleIntravenous Repeated Application of Low Dose ?-Galatosylceramide Boosts IFN-? Production in BALB/c Miceen_US
dc.titleIntravenous Repeated Application of Low Dose ?-Galatosylceramide Boosts IFN-? Production in BALB/c Mice
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu