Intravenous Repeated Application of Low Dose ?-Galatosylceramide Boosts IFN-? Production in BALB/c Mice
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Tarih
2012
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Akad Doktorlar Yayinevi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The aim of this study is to illustrate the relationship between Alpha Galatosylceramide (alpha-GalCer) dose and alpha-GalCer induced anergy of immune system in vivo. alpha-GalCer, a glycolipid and a widely studied immune modulator extracted from a marine sponge, shows its effect in vivo via presentation on CD1d molecule, MHC I like molecule bound glycolipid antigens, to NKT cells, a lymphocyte has both TCR and NK1.1, marker of T cells and NK cells respectively. The contradictory reports about alpha-GalCer are focused on two opposite axes of immune system: cancer and autoimmunity, failure and exaggeration of Th1 type immune response, respectively. In this study, the relation between the alpha-GalCer dose and the anergy was considered to clarify this dilemma. The groups of BALB/c mice were injected with various titer of alpha-GalCer intravenous way repeatedly and serum level of IFN-gamma was measured with mouse IFN-gamma ELISA. The Dramatic difference was found between groups after 2nd application of alpha-GalCer: Low dose alpha-GalCer boosted IFN-gamma; however, high dose, usual dose cited in literature, failed to induce IFN-gamma production. Besides, low concentrations of _-GalCer succeeded considerable amount of IFN-gamma in the serum after priming. This results show that minute level of alpha-GalCer can be recognized by NKT cells, and repeated in vivo application of low dose alpha-GalCer can improve Th1 capabilities of the immune system.
Açıklama
Anahtar Kelimeler
Nkt Cells, Galactosylceramide, Anergy, Th1
Kaynak
Uhod-Uluslararasi Hematoloji-Onkoloji Dergisi
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
22
Sayı
1
