The Effects of Silver Nanoparticles (AgNPs) on Thermophilic Bacteria: Antibacterial, Morphological, Physiological and Biochemical Investigations

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dc.contributor.authorJahan, Israt
dc.contributor.authorBekler, Fatma Matpan
dc.contributor.authorTunc, Ahmed
dc.contributor.authorGuven, Kemal
dc.date.accessioned2024-04-24T17:20:51Z
dc.date.available2024-04-24T17:20:51Z
dc.date.issued2024
dc.departmentDicle Üniversitesien_US
dc.description.abstractSince thermophilic microorganisms are valuable sources of thermostable enzymes, it is essential to recognize the potential toxicity of silver nanoparticles used in diverse industrial sectors. Thermophilic bacteria Geobacillus vulcani 2Cx, Bacillus licheniformis 3CA, Paenibacillus macerans 3CA1, Anoxybacillus ayderensis FMB1, and Bacillus paralicheniformis FMB2-1 were selected, and their MIC and MBC values were assessed by treatment with AgNPs in a range of 62.5-1500 mu g mL(-1). The growth inhibition curves showed that the G. vulcani 2Cx, and B. paralicheniformis FMB2-1 strains were more sensitive to AgNPs, demonstrating a reduction in population by 71.1% and 31.7% at 62.5 mu g mL(-1) and by 82.9% and 72.8% at 250 mu g mL(-1), respectively. TEM and FT-IR analysis revealed that AgNPs caused structural damage, cytoplasmic leakage, and disruption of cellular integrity. Furthermore, cell viability showed a significant decrease alongside an increase in superoxide radical (SOR; O-2(-)) production. beta-galactosidase biosynthesis decreased to 28.8% level at 500 mu g mL(-1) AgNPs for G. vulcani 2Cx, 32.2% at 250 mu g mL(-1) for A. ayderensis FMB1, and 38.8% only at 62.5 mu g mL(-1), but it was completely inhibited at 500 mu g mL(-1) for B. licheniformis 3CA. Moreover, B. paralicheniformis FMB2-1 showed a significant decrease to 11.2% at 125 mu g mL(-1). This study is the first to reveal the toxic effects of AgNPs on thermophilic bacteria.en_US
dc.description.sponsorshipDicle University Scientific Research Projects Coordinatorship (DUBAP)en_US
dc.description.sponsorshipWe also thank Filiz Koyuncu for providing technical support for the measurement of the FT-IR spectra.en_US
dc.identifier.doi10.3390/microorganisms12020402
dc.identifier.issn2076-2607
dc.identifier.issue2en_US
dc.identifier.pmid38399806
dc.identifier.scopus2-s2.0-85187300756
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.3390/microorganisms12020402
dc.identifier.urihttps://hdl.handle.net/11468/19278
dc.identifier.volume12en_US
dc.identifier.wosWOS:001177104300001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofMicroorganisms
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSilver Nanoparticlesen_US
dc.subjectAntibacterial Toxicityen_US
dc.subjectThermophilic Bacteriaen_US
dc.subjectBeta-Galactosidase Inhibitionen_US
dc.subjectSuperoxide Radicals (Sors)en_US
dc.subjectTemen_US
dc.subjectFt-Iren_US
dc.titleThe Effects of Silver Nanoparticles (AgNPs) on Thermophilic Bacteria: Antibacterial, Morphological, Physiological and Biochemical Investigationsen_US
dc.titleThe Effects of Silver Nanoparticles (AgNPs) on Thermophilic Bacteria: Antibacterial, Morphological, Physiological and Biochemical Investigations
dc.typeArticleen_US

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