BDNF and NGF gene polymorphisms and urine BDNF–NGF levels in children with primary monosymptomatic nocturnal enuresis

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dc.authorid0000-0001-6764-8336en_US
dc.contributor.authorEce, Aydın
dc.contributor.authorCoşkun, Salih
dc.contributor.authorŞahin, Cahit
dc.contributor.authorTan, İlhan
dc.contributor.authorKarabel, Duran
dc.contributor.authorÇim, Abdullah
dc.date.accessioned2023-11-03T10:56:04Z
dc.date.available2023-11-03T10:56:04Z
dc.date.issued2019en_US
dc.departmentDicle Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Çocuk Sağlığı ve Hastalıkları Ana Bilim Dalıen_US
dc.description.abstractIntroduction: The pathophysiology and genetic influences in nocturnal enuresis have not been fully elucidated. Delayed neuronal maturation has been suggested as a pathogenetic mechanism in primary monosymptomatic nocturnal enuresis (PMNE). Brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) are neurotrophins affecting maturation of the nervous system. Objective: The aim of this preliminary study was to investigate BDNF and NGF gene polymorphisms and urine levels of BDNF and NGF in children with PMNE as a first time. Study design: The single-nucleotide polymorphisms of BDNF (rs6265:G > A:Val66Met; rs8192466:C > T:Thr2Ile) and NGF (rs6330:C > T:Ala35Val, rs11466112:C > T:Arg221Trp) were investigated by comparing 104 children with PMNE and 140 healthy control subjects. Children with non-PMNE were excluded. DNA isolation and detection of polymorphisms were performed by real-time polymerase chain reaction. In addition, urine BDNF and NGF levels of 47 PMNE and 29 healthy children were measured by enzyme-linked immunosorbent assay method and normalized to urine creatinine (Cr) concentration for comparisons. Results: There were no differences in genotype and allele frequencies of BDNF rs6265 and NGF rs6330 polymorphisms between patients with PMNE and the control group (P > 0.05). No mutant alleles were found in BDNF rs8192466 and NGF rs11466112 polymorphisms in either group. Children with PMNE had higher urine BDNF/Cr (0.020 ± 0.010 vs 0.010 ± 0.002; P = 0.008) and NGF/Cr ratio (3.01 ± 1.87 pg/mg vs 1.77 ± 0.26 pg/mg; P = 0.002) compared with the control subjects. However, no significant differences were found in BDNF/Cr and NGF/Cr values between GG, GA, and AA genotypes of BDNF rs6265 polymorphism and CC and CT genotypes of NGF rs6330 polymorphism (P > 0.05). Discussion: In this study, no association of BDNF and NGF gene polymorphisms with PMNE was found, and urine neurotrophin concentrations were not directly influenced by investigated polymorphisms. Although, previously increased urine neurotrophin secretion has been found in detrusor overactivity, bladder inflammation, and dysfunctional voiding, this preliminary results also showed an increase in neurotrophins in PMNE. Higher urine neurotrophin levels may be related to delayed and continued neuronal maturation or increased production of neurotrophins in the bladder. The increased urine neurotrophins in PMNE may be an indicator of increased sensory nerve excitability of the bladder, contributing to the development of enuresis. Conclusion: This study showed that investigated neurotrophin gene polymorphisms did not make a significant contribution to the development of PMNE, but urine levels of neurotrophin gene products were higher in PMNE. Owing to the complexity and heterogeneity of genotype–phenotype relationships in enuresis, further studies are needed in PMNE.en_US
dc.description.sponsorshipDicle University 14-TF-103
dc.identifier.citationEce, A., Coşkun, S., Şahin, C., Tan, İ., Karabel, D. ve Çim, A. (2019). BDNF and NGF gene polymorphisms and urine BDNF–NGF levels in children with primary monosymptomatic nocturnal enuresis. Journal of Pediatric Urology, 15(3), 255.e1-255e7.en_US
dc.identifier.doi10.1016/j.jpurol.2019.03.010
dc.identifier.endpage255.e7en_US
dc.identifier.issn1477-5131
dc.identifier.issue3en_US
dc.identifier.pmid30981636
dc.identifier.scopus2-s2.0-85064073054
dc.identifier.scopusqualityQ2
dc.identifier.startpage255.e1en_US
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S1477513119300683?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/11468/13044
dc.identifier.volume15en_US
dc.identifier.wosWOS:000471774000025
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorCoşkun, Salih
dc.institutionauthorŞahin, Cahit
dc.institutionauthorTan, İlhan
dc.institutionauthorKarabel, Duran
dc.institutionauthorÇim, Abdullah
dc.institutionauthorEce, Aydın
dc.language.isoenen_US
dc.publisherElsevier Ltden_US
dc.relation.ispartofJournal of Pediatric Urology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.subjectNocturnal enuresisen_US
dc.subjectGenetic predisposition to diseaseen_US
dc.subjectBrain-derived neurotrophic factoren_US
dc.subjectNerve growth factoren_US
dc.subjectPolymorphismen_US
dc.titleBDNF and NGF gene polymorphisms and urine BDNF–NGF levels in children with primary monosymptomatic nocturnal enuresisen_US
dc.titleBDNF and NGF gene polymorphisms and urine BDNF–NGF levels in children with primary monosymptomatic nocturnal enuresis
dc.typeArticleen_US

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