Use of natural dye-casein complexes
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Tarih
2006
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The activity of proteolytic enzymes is commonly measured using casein as a substrate. A modified caseinolysis assay was developed with natural dyes such as juglone, lawsone, berberine, and quercetin for Subtilisin carlsberg , protease type XVI, and trypsin, respectively. The pH dependence and incubation time were determined. K-m, V-max, and k(cat)/K-m values were also determined for these enzymes. Lawsone was found to be a better substrate than the others.
Açıklama
Anahtar Kelimeler
Proteolytic Activity, Dyed-Casein, Natural Dyes, Soluble Substrates
Kaynak
Preparative Biochemistry & Biotechnology
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
36
Sayı
2
