Effects of trypan blue on corneal endothelium and anterior lens capsule in albino Wistar rats

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dc.contributor.authorAri, Seyhmus
dc.contributor.authorCaca, Ihsan
dc.contributor.authorUnlu, Kaan
dc.contributor.authorNergiz, Yusuf
dc.contributor.authorAksit, Ihsan
dc.date.accessioned2024-04-24T16:11:14Z
dc.date.available2024-04-24T16:11:14Z
dc.date.issued2006
dc.departmentDicle Üniversitesien_US
dc.description.abstractBackground: The development of continuous curvilinear capsulorrhexis (CCC) has contributed significantly to the tolerability and effectiveness of cataract extraction and intraocular lens implantation. Staining of the anterior capsule has become a popular method of increasing visibility when performing CCC. Objective: The aim of this study was to determine, using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), the highest concentrations of trypan blue dye that would not cause long-term toxicity after injection into the anterior chamber of rat eyes. Methods: The eyes of healthy female albino Wistar rats were used in this investigator-masked, controlled, 2-period, experimental study conducted over 12 weeks at the Dicle University Experimental Animal Laboratory, Diyarbakir, Turkey. The rats were randomly divided into 5 groups of 4 using a random-number table. Each rat was administered a 0.05-mL injection of trypan blue into the right eye in 1 of the following concentrations: 0.4%, 0.2%, 0.1%, 0.05%, or 0.025%. A 0.05-mL pH-balanced saline solution was injected into the left eye of each rat to act as a control. At 1 day after injection and 4 weeks after injection (early period), 1 rat from each concentration group was euthanized and their eyes were enucleated. At 12 weeks after injection (late period) the remaining 2 rats from each group were euthanized and their eyes were enucleated. Corneal endothelial cells and the anterior lens capsule of the enucleated eyes were analyzed using SEM and TEM, and the results were compared with those of the control group. In the TEM analysis, the primary end point was the histopathologic changes in the cellular organelles when compared with those in the control group. In the SEM analysis, the primary end point was changes in cell shape, intracellular junctions, and density of the microvilli when compared with those in the control group. Results: Forty eyes from 20 albino Wistar rats (mean [SD] age, 8.2 [1.6] weeks; mean [SD] weight, 175.6 [16.5] g) were used in the study. Each group of rats received a different concentration of trypan blue in the right eye. In the early period, both the 0.4% and 0.2% concentrations were associated with the impairment of the hexagonal structure of corneal endothelial cells and intercellular junctions. Those concentrations were also associated with an increased occurrence of cellular vacuolation, cytoplasmic edema, extensive granulation of the endoplasmic reticulum, pyknotic nuclei, and mitochondrial degeneration. In the late period, these changes were observed as persisting in a decreasing manner. With the 0.1% and 0.05% concentrations, the density of microvilli decreased, nuclei appeared normal, granulation of the endoplasmic reticulum and Golgi apparatus was active, and minimal levels of mitochondrial degeneration were observed. Conclusions: In this small experimental study in rat eyes, trypan blue at concentrations > 0.025% was associated with impaired morphology and structure of corneal endothelial cells after short-term exposure. This effect continued in a decreasing fashion after long-term exposure. No significant changes were noted in the control group or the group administered the 0.025% concentration.en_US
dc.identifier.doi10.1016/j.curtheres.2006.12.003
dc.identifier.endpage377en_US
dc.identifier.issn0011-393X
dc.identifier.issn1879-0313
dc.identifier.issue6en_US
dc.identifier.pmid24678109
dc.identifier.scopus2-s2.0-34249938931
dc.identifier.scopusqualityQ3
dc.identifier.startpage366en_US
dc.identifier.urihttps://doi.org/10.1016/j.curtheres.2006.12.003
dc.identifier.urihttps://hdl.handle.net/11468/15320
dc.identifier.volume67en_US
dc.identifier.wosWOS:000243764300001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofCurrent Therapeutic Research-Clinical and Experimental
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectTrypan Blueen_US
dc.subjectCorneal Endotheliumen_US
dc.subjectLens Epitheliumen_US
dc.subjectCapsulorrhexisen_US
dc.titleEffects of trypan blue on corneal endothelium and anterior lens capsule in albino Wistar ratsen_US
dc.titleEffects of trypan blue on corneal endothelium and anterior lens capsule in albino Wistar rats
dc.typeArticleen_US

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