Production of lipase by a newly isolated Bacillus coagulans under solid-state fermentation using melon wastes

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dc.contributor.authorAlkan, Huseyin
dc.contributor.authorBaysal, Zubeyde
dc.contributor.authorUyar, Fikret
dc.contributor.authorDogru, Mehmet
dc.date.accessioned2024-04-24T15:59:38Z
dc.date.available2024-04-24T15:59:38Z
dc.date.issued2007
dc.departmentDicle Üniversitesien_US
dc.description.abstractAn extracellular lipase was produced by Bacillus coagulans by solid-state fermentation. Solid waste from melon was used as the basic nutrient source and was supplemented with olive oil. The highest lipase production (78,069 U/g) was achieved after 24 h of cultivation with 1% olive oil enrichment. Enzyme had an optimal activity at 37 degrees C and pH 7.0, and sodium dodecyl sulfate increased lipase activity. NH4NO3 increased enzyme production, whereas organic nitrogen had no effect. The effect of the type of carbon sources on lipolytic enzyme production was also studied. The best results were obtained with starch and maltose (148,932 and 141,629 U/g, respectively), whereas a rather low enzyme activity was found in cultures grown on glucose and galactose (approx 118,769 and 123,622 U/g, respectively). Enzyme was inhibited with Mn+2 and Ni+2 by 68 and 74%, respectively. By contrast, Ca+2 enhanced enzyme production by 5%.en_US
dc.identifier.doi10.1007/BF02686016
dc.identifier.endpage192en_US
dc.identifier.issn0273-2289
dc.identifier.issue2en_US
dc.identifier.pmid17496339
dc.identifier.scopus2-s2.0-34248350688
dc.identifier.scopusqualityQ2
dc.identifier.startpage183en_US
dc.identifier.urihttps://doi.org/10.1007/BF02686016
dc.identifier.urihttps://hdl.handle.net/11468/14185
dc.identifier.volume136en_US
dc.identifier.wosWOS:000245023200004
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherHumana Press Incen_US
dc.relation.ispartofApplied Biochemistry and Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSolid-State Fermentationen_US
dc.subjectBacillusen_US
dc.subjectLipase Productionen_US
dc.titleProduction of lipase by a newly isolated Bacillus coagulans under solid-state fermentation using melon wastesen_US
dc.titleProduction of lipase by a newly isolated Bacillus coagulans under solid-state fermentation using melon wastes
dc.typeArticleen_US

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