The effects of amino acids on production and transport of α-amylase through bacterial membranes

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Küçük Resim

Tarih

2000

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Wiley-VCH Verlag

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

Bacillus subtilis was grown in media containing various amino acids. The effects of these aminoacids on the production of secreted alpha-amylase and membrane-bound alpha-amylase were investigated. Their effects on the transport of alpha-amylase through membranes were also determined. The synthesis rate of alpha-amylase isolated from bacterial membranes was stimulated by growth in media separately containing Ala and Arg, all other amino acids being inhibitory. Cys, Arg and Leu increased the transport of alpha-amylase through the membrane, while Ala, Asp and Ser inhibited it. Secreted alpha-amylase activity was appreciably stimulated by Ala, Arg, Gin, Gly, Leu, Phe, Pro, and especially Cys while it was repressed by Asp, Asn, Glu, Lys, Met, Ser, Thr and Trp.

Açıklama

Anahtar Kelimeler

Bacillus subtilis, Alpha-amylase, Membrane proteins and transport, Enzyme structure and mechanisms, Amino acids, Enzyme inhibition

Kaynak

Starch-Starke

WoS Q Değeri

Q3

Scopus Q Değeri

Q2

Cilt

52

Sayı

8-9

Künye

Agüloǧlu, S., Ensari, N. Y., Uyar, F. ve Otludil, B. (2000). The effects of amino acids on production and transport of α-amylase through bacterial membranes. Starch-Starke, 52(8-9), 290-295.