UHPLC-ESI-MS/MS and GC-MS Analyses on Phenolic, Fatty Acid and Essential Oil of Verbascum pinetorum with Antioxidant, Anticholinesterase, Antimicrobial and DNA Damage Protection Effects

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dc.contributor.authorBoga, Mehmet
dc.contributor.authorErtas, Abdulselam
dc.contributor.authorYilmaz, Mustafa Abdullah
dc.contributor.authorKizil, Murat
dc.contributor.authorCeken, Bircan
dc.contributor.authorHasimi, Nesrin
dc.contributor.authorOzden, Tugba Yilmaz
dc.date.accessioned2024-04-24T17:40:00Z
dc.date.available2024-04-24T17:40:00Z
dc.date.issued2016
dc.departmentDicle Üniversitesien_US
dc.description.abstractThis paper is the first phytochemical and ABTS cation radical decolorisation activity, cupric reducing antioxidant capacity, anticholinesterase and DNA damage protection effect of endemic Verbascum pinetorum (Boiss.) O. Kuntze. Phenolic profile of V. pinetorum were qualified and quantified by UHPLC-ESI-MS/MS analysis. Malic acid (47250.61 +/- 2504.28 mu g/g) and luteolin (7651.96 +/- 527.98 mu g/g) were found as most abundant compounds for metanol and acetone extracts, respectively. Fatty acid and essential oil compositions were determined by GC-MS analysis. The main components of fatty acid were found to be palmitic (27.1%) and stearic (22.1%) acids. The main compounds of the essential oil were cineole (16.9%) and alpha-selinene (16.4%). The acetone extract was found to be more active than BHT used as a standard in beta-carotene-linoleic acid test system. In DPPH free radical scavenging activity, the acetone and methanol extracts showed higher activity than BHT at all tested concentrations. The acetone, methanol and water extracts showed strong inhibition while the acetone extract showed better activity than BHT and a-tocopherol which were used as standards in ABTS cation radical scavenging and cupric reducing antioxidant capacity assays, respectively. All extracts were found to be inactive in antialzheimer activity. The acetone extract exhibited moderate antimicrobial activity against C. albicans. The methanol extract of V. pinetorum were found no significant effect on DNA cleavage protection.en_US
dc.description.sponsorshipDicle University Science and Technology Research and Application Center (DUBTAM)en_US
dc.description.sponsorshipWe thank to Dicle University Science and Technology Research and Application Center (DUBTAM) for the partial support of this study.en_US
dc.identifier.endpage405en_US
dc.identifier.issn1735-0328
dc.identifier.issn1726-6890
dc.identifier.issue3en_US
dc.identifier.pmid27980574
dc.identifier.scopus2-s2.0-84990026502
dc.identifier.scopusqualityQ2
dc.identifier.startpage393en_US
dc.identifier.urihttps://hdl.handle.net/11468/21512
dc.identifier.volume15en_US
dc.identifier.wosWOS:000391797700016
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherShaheed Beheshti Univ, Sch Pharmacyen_US
dc.relation.ispartofIranian Journal of Pharmaceutical Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectVerbascum Pinetorumen_US
dc.subjectFatty Aciden_US
dc.subjectEssential Oilen_US
dc.subjectAntioxidanten_US
dc.subjectAnticholinesteraseen_US
dc.subjectAntimicrobialen_US
dc.subjectDna Cleavage Protectionen_US
dc.titleUHPLC-ESI-MS/MS and GC-MS Analyses on Phenolic, Fatty Acid and Essential Oil of Verbascum pinetorum with Antioxidant, Anticholinesterase, Antimicrobial and DNA Damage Protection Effectsen_US
dc.titleUHPLC-ESI-MS/MS and GC-MS Analyses on Phenolic, Fatty Acid and Essential Oil of Verbascum pinetorum with Antioxidant, Anticholinesterase, Antimicrobial and DNA Damage Protection Effects
dc.typeArticleen_US

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