Improved sensitivity of nucleic acid amplification for rapid diagnosis of tuberculous meningitis

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dc.contributor.authorJohansen, IS
dc.contributor.authorLundgren, B
dc.contributor.authorTabak, F
dc.contributor.authorPetrini, B
dc.contributor.authorHosoglu, S
dc.contributor.authorSaltoglu, N
dc.contributor.authorThomsen, VO
dc.date.accessioned2024-04-24T17:11:52Z
dc.date.available2024-04-24T17:11:52Z
dc.date.issued2004
dc.departmentDicle Üniversitesien_US
dc.description.abstractEarly diagnosis of tuberculous meningitis (TBM) is essential for a positive outcome; but present microbiological diagnostic techniques are insensitive, slow, or laborious. We evaluated the standard BDProbeTec ET strand displacement amplification method (the standard ProbeTec method) for the detection of Mycobacterium tuberculosis complex organisms in parallel with the ProbeTec method with a modified pretreatment procedure with 101 prospectively collected cerebrospinal fluid specimens from 94 patients with suspected TBM. By the modified method, the sample-washing step was omitted. A definitive diagnosis was attained by culture. Thirteen specimens from 12 patients were culture positive for M. tuberculosis complex organisms; three specimens (23%) were microscopy positive for acid-fast bacilli. Among the culture-positive specimens, the standard ProbeTec method was positive for 8 (61.5%) and the modified assay was positive for 10 (76.9%). The overall specificity by both procedures was 98.8% compared to the results of culture. After discrepancy analysis, conducted by reviewing the patients' previous laboratory data, the specificity increased to 100%. If the cutoff value for respiratory specimens was adjusted from the recommended value of 3,400 to 1,000, the sensitivity of the modified procedure increased to 84.7%, with unchanged specificity. Results were obtained in 3 to 4 h. The new pretreatment procedure with the ProbeTec assay described here provides a rapid, simple, and sensitive tool for the diagnosis of TBM.en_US
dc.identifier.doi10.1128/JCM.42.7.3036-3040.2004
dc.identifier.endpage3040en_US
dc.identifier.issn0095-1137
dc.identifier.issue7en_US
dc.identifier.pmid15243056
dc.identifier.scopus2-s2.0-3142745257
dc.identifier.scopusqualityQ1
dc.identifier.startpage3036en_US
dc.identifier.urihttps://doi.org/10.1128/JCM.42.7.3036-3040.2004
dc.identifier.urihttps://hdl.handle.net/11468/17759
dc.identifier.volume42en_US
dc.identifier.wosWOS:000222672100025
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherAmer Soc Microbiologyen_US
dc.relation.ispartofJournal of Clinical Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject[No Keyword]en_US
dc.titleImproved sensitivity of nucleic acid amplification for rapid diagnosis of tuberculous meningitisen_US
dc.titleImproved sensitivity of nucleic acid amplification for rapid diagnosis of tuberculous meningitis
dc.typeArticleen_US

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