Adding hormones sequentially could be an effective approach for IVM of dog oocytes

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dc.contributor.authorEvecen, M.
dc.contributor.authorCirit, Ue
dc.contributor.authorDemir, K.
dc.contributor.authorHamzaoglu, A. I.
dc.contributor.authorBakirer, G.
dc.contributor.authorPabuccuoglu, S.
dc.contributor.authorBirler, S.
dc.date.accessioned2024-04-24T16:18:28Z
dc.date.available2024-04-24T16:18:28Z
dc.date.issued2011
dc.departmentDicle Üniversitesien_US
dc.description.abstractThere have not been successful and repeatable methods for in vitro embryo production in the dog. Up to date, only one blactocyst has been achieved on in vitro culture. Since reproductive physiology of the dog is different from that of other mammalian species, it seems that a suitable method for in vitro production of canine embryos is still far from being designed and routinely applied, and an effective protocol is needed. Therefore, the aim of the present study was to examine the effects of adding hormones sequentially, for mimicking the dog's in vivo endocrine milieu, on maturation of immature dog oocytes in vitro. At the end of the 96 h IVM period, nuclear maturation rates were evaluated by the aceto-orcein staining method. In comparison relating IVM rates, the sequential hormone addition was more beneficial on IVM rates (MI + MII) than the traditional hormone addition and control groups (48.1%, 38.9% and 23.0% respectively; P < 0.0001). As a result, hormone addition sequentially may be an effective approach for the IVM of the immature dog oocytes. We suggest that attempts to define the adequate conditions for IVM in the dog should extend towards this new perspective. (C) 2011 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipIstanbul University [1145]en_US
dc.description.sponsorshipThe present work was supported by the Research Fund of Istanbul University. Project No: 1145.en_US
dc.identifier.doi10.1016/j.theriogenology.2011.01.004
dc.identifier.endpage1651en_US
dc.identifier.issn0093-691X
dc.identifier.issn1879-3231
dc.identifier.issue9en_US
dc.identifier.pmid21396692
dc.identifier.scopus2-s2.0-79961167258
dc.identifier.scopusqualityQ1
dc.identifier.startpage1647en_US
dc.identifier.urihttps://doi.org/10.1016/j.theriogenology.2011.01.004
dc.identifier.urihttps://hdl.handle.net/11468/16119
dc.identifier.volume75en_US
dc.identifier.wosWOS:000290925500009
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofTheriogenology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDogen_US
dc.subjectOocyteen_US
dc.subjectIvmen_US
dc.subjectHormoneen_US
dc.titleAdding hormones sequentially could be an effective approach for IVM of dog oocytesen_US
dc.titleAdding hormones sequentially could be an effective approach for IVM of dog oocytes
dc.typeArticleen_US

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