Proteolytic activity of commercial subtilisin from genus Bacillus on some animals milk caseins

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dc.contributor.authorAytekin, C
dc.contributor.authorKaya, Z
dc.contributor.authorDogru, M
dc.date.accessioned2024-04-24T17:37:52Z
dc.date.available2024-04-24T17:37:52Z
dc.date.issued1997
dc.departmentDicle Üniversitesien_US
dc.description.abstractCasein is a non-specific substrate for the all proteases. In this study casein was purified from cow, water buffalo, sheep and goat milk and used as a substrate for subtilisin purified preparations from genus Bacillus. On the basis of K-M and V-max value we can say that cow milk casein is the best substrate for the enzyme compared to other animals milk caseins. On the other hand, we have investigated the possible relationship between the Ca2+ and (PO4)(3-) content of casein and the casein appropriate for the enzyme. The cow milk casein with lowermost Ca2+/(PO4)(3-) ratio suggests that these ions may have an influence on enzyme-substrate interaction. Although this enzyme is active in the pH range of 7-11 and a temperature range of 25-60 degrees C, the optimal pH and temperature for the enzyme against cow milk casein were found to be 10,0 and 37 degrees C respectively.en_US
dc.identifier.endpage236en_US
dc.identifier.issn0749-5331
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-0031464497
dc.identifier.scopusqualityN/A
dc.identifier.startpage229en_US
dc.identifier.urihttps://hdl.handle.net/11468/21226
dc.identifier.volume13en_US
dc.identifier.wosWOS:A1997YK00700004
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherMbr Press Incen_US
dc.relation.ispartofBiochemical Archives
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject[No Keyword]en_US
dc.titleProteolytic activity of commercial subtilisin from genus Bacillus on some animals milk caseinsen_US
dc.titleProteolytic activity of commercial subtilisin from genus Bacillus on some animals milk caseins
dc.typeArticleen_US

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