Proteolytic activity of commercial subtilisin from genus Bacillus on some animals milk caseins
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Tarih
1997
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Mbr Press Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Casein is a non-specific substrate for the all proteases. In this study casein was purified from cow, water buffalo, sheep and goat milk and used as a substrate for subtilisin purified preparations from genus Bacillus. On the basis of K-M and V-max value we can say that cow milk casein is the best substrate for the enzyme compared to other animals milk caseins. On the other hand, we have investigated the possible relationship between the Ca2+ and (PO4)(3-) content of casein and the casein appropriate for the enzyme. The cow milk casein with lowermost Ca2+/(PO4)(3-) ratio suggests that these ions may have an influence on enzyme-substrate interaction. Although this enzyme is active in the pH range of 7-11 and a temperature range of 25-60 degrees C, the optimal pH and temperature for the enzyme against cow milk casein were found to be 10,0 and 37 degrees C respectively.
Açıklama
Anahtar Kelimeler
[No Keyword]
Kaynak
Biochemical Archives
WoS Q Değeri
Q4
Scopus Q Değeri
N/A
Cilt
13
Sayı
4
