Evaluation of gene expression levels in the diagnosis of lung adenocarcinoma and malignant pleural mesothelioma

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dc.contributor.authorOmeroglu, Gokcen Simsek
dc.contributor.authorAgababaoglu, Ismail
dc.contributor.authorDursun, Duygu
dc.contributor.authorOzekinci, Selver
dc.contributor.authorErcetin, Pinar
dc.contributor.authorEllidokuz, Hulya
dc.contributor.authorAktas, Safiye
dc.date.accessioned2024-04-24T17:28:01Z
dc.date.available2024-04-24T17:28:01Z
dc.date.issued2020
dc.departmentDicle Üniversitesien_US
dc.description.abstractBackground: This study aims to evaluate gene expression levels in the diagnosis of lung adenocarcinoma and malignant pleural mesothelioma both which have a distinct treatment and prognosis. Methods: Between January 2012 and January 2014, 12 newly diagnosed patients with a lung adenocarcinoma, 12 patients with malignant pleural mesothelioma, and eight healthy individuals as the control group were included. After treatment of the fresh samples of lung adenocarcinoma stored at -80 degrees C for ribonucleic acid isolation, and paraffin-embedded tissues of patients with malignant pleural mesothelioma were deparaffinized, complementary deoxyribonucleic acid synthesis and expression of 84 genes associated with deoxyribonucleic acid repair were analyzed via real-time polymerase chain reaction assay. According to the expression of tumor cells, expression of each fold change was calculated. Results: The BRCA1, BRCA2, CDK7, MLH3, MSH4, NEIL3, SMUG1, UNG, XRCC2, and XRCC4 genes showed more than five-fold higher expression in the patients with lung adenocarcinomas, compared to the control group. The patients with malignant pleural mesothelioma showed a five-fold higher expression in the APEX2, BRCA1, BRCA2, CDK7, MLH1, MLH3, MSH3, MSH4, NEIL3, PARP2, PARP3, PMS1, RAD50, RAD51, RAD51B, RAD51D, RAD52, RPA3, SMUG1, UNG, XPA, XRCC2, and XRCC4 genes, compared to the control group. Comparing malignant pleural mesothelioma with lung adenocarcinoma cases, we found that CDK7, MLH1, TREX1, PRKDC, XPA, PMS1, UNG, and RPA3 genes were overexpressed. Conclusion: Our study results showed differences between expression profiles of deoxyribonucleic acid repair genes in lung adenocarcinoma and malignant pleural mesothelioma cells. Based on our study results, we suggest that TREX1, PRKDC, and PMS1 genes may play a key role in the differential diagnosis of these two entities.en_US
dc.identifier.doi10.5606/tgkdc.dergisi.2020.17279
dc.identifier.endpage196en_US
dc.identifier.issn1301-5680
dc.identifier.issue1en_US
dc.identifier.pmid32175161
dc.identifier.startpage188en_US
dc.identifier.trdizinid375501
dc.identifier.urihttps://doi.org/10.5606/tgkdc.dergisi.2020.17279
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/375501
dc.identifier.urihttps://hdl.handle.net/11468/20297
dc.identifier.volume28en_US
dc.identifier.wosWOS:000512647300023
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherBaycinar Medical Publ-Baycinar Tibbi Yayinciliken_US
dc.relation.ispartofTurk Gogus Kalp Damar Cerrahisi Dergisi-Turkish Journal of Thoracic and Cardiovascular Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAdenocarcinomaen_US
dc.subjectGene Expressionen_US
dc.subjectLungen_US
dc.subjectMalignanten_US
dc.subjectMesotheliomaen_US
dc.titleEvaluation of gene expression levels in the diagnosis of lung adenocarcinoma and malignant pleural mesotheliomaen_US
dc.titleEvaluation of gene expression levels in the diagnosis of lung adenocarcinoma and malignant pleural mesothelioma
dc.typeArticleen_US

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