Targeted disruption of homoserine dehydrogenase gene and its effect on cephamycin C production in Streptomyces clavuligerus

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dc.contributor.authorYilmaz, Ebru I.
dc.contributor.authorCaydasi, Ayse K.
dc.contributor.authorOezcengiz, Guelay
dc.date.accessioned2024-04-24T16:02:04Z
dc.date.available2024-04-24T16:02:04Z
dc.date.issued2008
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe aspartate pathway of Streptomyces clavuligerus is an important primary metabolic pathway which provides substrates for beta-lactam synthesis. In this study, the hom gene which encodes homoserine dehydrogenase was cloned from the cephamycin C producer S. clavuligerus NRRL 3585 and characterized. The fully sequenced open reading frame encodes 433 amino acids with a deduced M (r) of 44.9 kDa. The gene was heterologously expressed in the auxotroph mutant Escherichia coli CGSC 5075 and the recombinant protein was purified. The cloned gene was used to construct a plasmid containing a hom disruption cassette which was then transformed into S. clavuligerus. A hom mutant of S. clavuligerus was obtained by insertional inactivation via double crossover, and the effect of hom gene disruption on cephamycin C yield was investigated by comparing antibiotic levels in culture broths of this mutant and in the parental strain. Disruption of hom gene resulted in up to 4.3-fold and twofold increases in intracellular free L-lysine concentration and specific cephamycin C production, respectively, during stationary phase in chemically defined medium.en_US
dc.identifier.doi10.1007/s10295-007-0259-8
dc.identifier.endpage7en_US
dc.identifier.issn1367-5435
dc.identifier.issn1476-5535
dc.identifier.issue1en_US
dc.identifier.pmid17909870
dc.identifier.scopus2-s2.0-36849053355
dc.identifier.scopusqualityQ1
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1007/s10295-007-0259-8
dc.identifier.urihttps://hdl.handle.net/11468/14603
dc.identifier.volume35en_US
dc.identifier.wosWOS:000251487300001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherSpringer Heidelbergen_US
dc.relation.ispartofJournal of Industrial Microbiology & Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectStreptomyces Clavuligerusen_US
dc.subjectHomoserine Dehydrogenaseen_US
dc.subjectCephamycin Cen_US
dc.subjectAspartate Pathwayen_US
dc.subjectGene Disruptionen_US
dc.titleTargeted disruption of homoserine dehydrogenase gene and its effect on cephamycin C production in Streptomyces clavuligerusen_US
dc.titleTargeted disruption of homoserine dehydrogenase gene and its effect on cephamycin C production in Streptomyces clavuligerus
dc.typeArticleen_US

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