The effects of caffeic acid phenethyl ester in acute methanol toxicity on rat retina and optic nerve

Basit Öğe Kaydı
dc.contributor.authorSahin, Alparslan
dc.contributor.authorKaya, Savas
dc.contributor.authorTurkcu, Gul
dc.contributor.authorCingu, Abdullah Kursat
dc.contributor.authorYuksel, Harun
dc.contributor.authorTurkcu, Fatih Mehmet
dc.contributor.authorYuksel, Hatice
dc.date.accessioned2024-04-24T17:20:35Z
dc.date.available2024-04-24T17:20:35Z
dc.date.issued2013
dc.departmentDicle Üniversitesien_US
dc.description.abstractPurpose: We aimed to test caffeic acid phenethyl ester (CAPE) as an antidote for acute methanol (MeOH) toxicity and to compare it with ethanol. Methods: This study included five groups, each containing eight rats. The groups were control, methotrexate (MTX), MeOH, ethanol and CAPE. All rats except control group were treated with intraperitoneal (i.p.) MTX (0.3 mg/kg/d) for 7 d. At the 8th day of the experiment, i.p. injection of MeOH (3 g/kg) was administered in MeOH, ethanol and CAPE groups. Four hours after MeOH treatment, 0.5 g/kg ethanol was injected i.p. in ethanol group; 10 mu mol/kg CAPE i.p. in CAPE group; serum physiologic i.p. in other groups. After 8 h, rats were anaesthetized and sacrificed. Total anti-oxidant status (TAS), total oxidant status (TOS) were measured on the dissected and excised retina and optic nerve samples. Fellow eyes were used for histopathologic evaluation and the cell count of retinal ganglion cell (RGC) layer. In addition, interactions of alcohol dehydrogenase with CAPE, ethanol, MeOH and pyrazole derivatives were investigated. Results: Either CAPE or ethanol co-treatment decreased the TOS levels and increased the TAS levels compared to the MeOH group. MeOH treatment decreased the mean cell count in RGC layer. CAPE co-treatment significantly prevented cell loss (p=0.040). Besides, in silico calculations showed that binding affinity of CAPE to alcohol dehydrogenase was higher than those of MeOH, ethanol, and pyrazole derivatives were. Conclusion: This study demonstrated that CAPE treatment decreased the oxidative stress in acute MeOH intoxication in the retina and optic nerve; beside that, protected RGC layer histology. In silico, CAPE had higher affinity score than MeOH, ethanol, pyrazole and pyrazole derivatives in the case of interaction with alcohol dehydrogenase.en_US
dc.identifier.doi10.3109/15569527.2013.771642
dc.identifier.endpage267en_US
dc.identifier.issn1556-9527
dc.identifier.issn1556-9535
dc.identifier.issue4en_US
dc.identifier.pmid23461623
dc.identifier.scopus2-s2.0-84883155350
dc.identifier.scopusqualityQ3
dc.identifier.startpage263en_US
dc.identifier.urihttps://doi.org/10.3109/15569527.2013.771642
dc.identifier.urihttps://hdl.handle.net/11468/19140
dc.identifier.volume32en_US
dc.identifier.wosWOS:000323527300001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofCutaneous and Ocular Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCaffeic Acid Phenethyl Esteren_US
dc.subjectEthanolen_US
dc.subjectMethanolen_US
dc.subjectNeuropathyen_US
dc.subjectOxidative Stressen_US
dc.subjectRetinopathyen_US
dc.subjectToxicityen_US
dc.titleThe effects of caffeic acid phenethyl ester in acute methanol toxicity on rat retina and optic nerveen_US
dc.titleThe effects of caffeic acid phenethyl ester in acute methanol toxicity on rat retina and optic nerve
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu