Single-strand conformation polymorphism-based genetic characterization of the Cyclospora cayetanensis strains collected from different provinces in Turkey

Basit Öğe Kaydı
dc.contributor.authorCicek, Muttalip
dc.contributor.authorYildirim, Ibrahim Halil
dc.contributor.authorCengiz, Zeynep Tas
dc.contributor.authorKaraman, Ulku
dc.date.accessioned2024-04-24T17:20:22Z
dc.date.available2024-04-24T17:20:22Z
dc.date.issued2021
dc.departmentDicle Üniversitesien_US
dc.description.abstractIntroduction and objective. Cyclospora cayetanensis, a coccidian protozoan species, has been recently found to cause diarrhea in all age groups in immunocompetent and immunocompromised individuals in most regions of the world. This study aimed to conduct the molecular detection of C. cayetanensis and to determine the genetic diversity of the 18S ribosomal RNA (rRNA) gene sequence of C. cayetanensis isolated from individuals living in different provinces in Turkey by using PCR-single-strand conformation polymorphism (SSCP). Materials and method. A total of 22 subjects were included in the study. Fourteen of the subjects were female and eight were male, with ages ranging between 7-65 years. Stool specimens were examined using wet mount and modified acid-fast staining methods, which revealed the presence of oocysts in the samples. The 18S rRNA ITS-1 Ccits37f-GCTTGCTATGTTTTAGCATGTGG and Ccits501r-GCACAATGAATGCACACACA gene regions were used as primers. The PCR products were analyzed by agarose gel electrophoresis and visualized on a UV transilluminator. For the SSCP, the PCR products were denatured with formamide, run for 16 h in 6% (49:1) polyacrylamide gel, and then imaged with silver staining. Results. SSCP assay was performed given that the DNA strands demonstrated different folds; the DNA strands contain different nucleotides based on the PCR-SSCP results for the Cyclospora strains collected in 4 provinces. Moreover, 3 different band profiles were observed in the investigated samples. A slight mutation difference was observed among the strains collected. Conclusions. Further comprehensive studies involving more C. cayetanensis-positive specimens and utilizing different mutation screening methods are warranted to demonstrate mutation differences in Cyclopora strains in Turkey.en_US
dc.description.sponsorshipDicle University in Diyarbakir, Turkey, Scientific Research Coordinatorship (DUBAP) [11-TF-58]en_US
dc.description.sponsorshipThe study was supported by Dicle University in Diyarbakir, Turkey, Scientific Research Coordinatorship (DUBAP), Project No. 11-TF-58.en_US
dc.identifier.doi10.26444/aaem/136116
dc.identifier.endpage270en_US
dc.identifier.issn1232-1966
dc.identifier.issn1898-2263
dc.identifier.issue2en_US
dc.identifier.pmid34184509
dc.identifier.scopus2-s2.0-85108681593
dc.identifier.scopusqualityQ2
dc.identifier.startpage267en_US
dc.identifier.urihttps://doi.org/10.26444/aaem/136116
dc.identifier.urihttps://hdl.handle.net/11468/18989
dc.identifier.volume28en_US
dc.identifier.wosWOS:000664478800012
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherInst Agricultural Medicineen_US
dc.relation.ispartofAnnals of Agricultural and Environmental Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCyclospora Cayetanensisen_US
dc.subjectMolecular Characterizationen_US
dc.subjectTurkiyeen_US
dc.titleSingle-strand conformation polymorphism-based genetic characterization of the Cyclospora cayetanensis strains collected from different provinces in Turkeyen_US
dc.titleSingle-strand conformation polymorphism-based genetic characterization of the Cyclospora cayetanensis strains collected from different provinces in Turkey
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu