Analysis of bioactive compounds using LC-ESI-MS/MS, cytotoxic, antimicrobial effects, and enzyme activities from Cyclotrichium origanifolium

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dc.contributor.authorAktepe, Necmettin
dc.contributor.authorBaran, Ayse
dc.contributor.authorAtalar, Mehmet Nuri
dc.contributor.authorBaran, Mehmet Firat
dc.contributor.authorKeskin, Cumali
dc.contributor.authorTaskin, Abdullah
dc.contributor.authorYavuz, Oemer
dc.date.accessioned2024-04-24T17:11:25Z
dc.date.available2024-04-24T17:11:25Z
dc.date.issued2023
dc.departmentDicle Üniversitesien_US
dc.description.abstractCyclotrichium origanifolium is a medicinal plant belonging to the Lamiaceae family. In this study, phenolic content analysis, antimicrobial effects, and cytotoxic effects of extracts of C. origanifolium were investigated. In the extracts, phenolic compound analysis by the liquid chromatography-electrospray ionization-tandem mass spectrometry method, antimicrobial effect by the minimum inhibition concentration method, and cytotoxic effect on human dermal fibroblasts (HDF), glioblastoma cell (U87), ovarian adenocarcinoma cell (Skov-3), and human colorectal adenocarcinoma cell (CaCo-2) cancer cell lines were investigated. Cytotoxicity analyses were performed by the MTT method. In addition, the GST and AChE enzyme activities of the extracts were also measured. Around 18 compounds were detected in both the methanol and ethanol extract. It was found that the best antimicrobial effect on Gram-negative Pseudomonas aeruginosa was on methanol extract, while the ethanol extract was on Candida albicans fungus (respectively, 2.50 mg/ml, 5.0 mu g/ml). A 500 mu g/ml of methanol extract has been shown to have cytotoxic activity high effect on HDF cells. GST and AChE activity were found to decrease in a concentration-dependent manner.en_US
dc.description.sponsorshipMardin Artuklu University Scientific Project Coordination Unit; [MAU.BAP.22.SBF.002]en_US
dc.description.sponsorshipThe present study was supported by Mardin Artuklu University Scientific Project Coordination Unit, Grant number: MAU.BAP.22.SBF.002. LC-ESI-MS/MS analyses included in the study were carried out at Igdir University Research Laboratory Application and Research Center (ALUM).en_US
dc.identifier.doi10.1111/cbdd.14177
dc.identifier.endpage748en_US
dc.identifier.issn1747-0277
dc.identifier.issn1747-0285
dc.identifier.issue3en_US
dc.identifier.pmid36366959
dc.identifier.scopus2-s2.0-85143424960
dc.identifier.scopusqualityQ2
dc.identifier.startpage740en_US
dc.identifier.urihttps://doi.org/10.1111/cbdd.14177
dc.identifier.urihttps://hdl.handle.net/11468/17505
dc.identifier.volume101en_US
dc.identifier.wosWOS:000890248500001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofChemical Biology & Drug Design
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAntimicrobialen_US
dc.subjectAntioxidanten_US
dc.subjectCyclotricium Origanifoliumen_US
dc.subjectCytotoxicen_US
dc.subjectPhenolicsen_US
dc.titleAnalysis of bioactive compounds using LC-ESI-MS/MS, cytotoxic, antimicrobial effects, and enzyme activities from Cyclotrichium origanifoliumen_US
dc.titleAnalysis of bioactive compounds using LC-ESI-MS/MS, cytotoxic, antimicrobial effects, and enzyme activities from Cyclotrichium origanifolium
dc.typeArticleen_US

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