LPS-induced Src family kinases activity mediates IL-10 production through activation of STAT3 in peripheral blood mononuclear cells of patients with Behcet's Disease

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dc.contributor.authorIrtegun-Kandemir, S.
dc.contributor.authorTekin, M. A.
dc.contributor.authorBozkurt, M.
dc.contributor.authorDagli, A. Z.
dc.contributor.authorKalkanli-Tas, S.
dc.date.accessioned2024-04-24T17:17:57Z
dc.date.available2024-04-24T17:17:57Z
dc.date.issued2017
dc.departmentDicle Üniversitesien_US
dc.description.abstractBehcet's disease (BD) is a chronic inflammatory disorder characterized by recurrent oral and genital ulcers, uveitis and skin lesions. Although, the pathogenesis of BD remains poorly understood, excessive or dysregulated cytokine production including IL-10 is associated with BD. Revealing the key molecular mechanism by which IL-10 expression is regulated is crucial to understanding the pathogenesis of BD. The aim of this study was to investigate whether Src family kinases (SFKs) are upstream mediators of STAT3/IL-10 pathway in peripheral blood mono nuclear cells (PBMCs) of active BD patients. Twenty active BD patients and twenty healthy subjects used as control were included in the study. PBMCs were isolated from total blood by density gradient centrifugation. Western blot and ELISA methods were applied to analyze lipopolysaccharide (LPS)-induced SFKs/STAT3/IL10 signaling pathway in BD. Inhibition of SFKs activity suppressed LPS-induced IL-10 production in PBMCs from both controls and active BD patients. Similarly, blockage of STAT3 activation abrogated LPS-induced IL-10 production. However, LPS-induced STAT3 activation required for IL-10 production was found to be dependent on SFKs activity as LPS-induced STAT3 phosphorylation was reduced by the inhibition of SFKs activity in PBMCs of active BD patients. SFKs activity is essential for LPS-induced STAT3/IL-10 pathway in PBMCs of active BD patients. Manipulation of the SFKs activity may offer a novel therapeutic approach for BD.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey [213S016]en_US
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkey project 213S016. We thank to Fatih M Turkcu and Zeynep M Akkurt for helping with some of the patients samples.en_US
dc.identifier.doi10.14715/cmb/2017.63.10.18
dc.identifier.endpage121en_US
dc.identifier.issn0145-5680
dc.identifier.issn1165-158X
dc.identifier.issue10en_US
dc.identifier.pmid29096751en_US
dc.identifier.scopus2-s2.0-85032742484en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage116en_US
dc.identifier.urihttps://doi.org/10.14715/cmb/2017.63.10.18
dc.identifier.urihttps://hdl.handle.net/11468/18468
dc.identifier.volume63en_US
dc.identifier.wosWOS:000418156300018
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherC M B Assocen_US
dc.relation.ispartofCellular and Molecular Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBehcet's Diseaseen_US
dc.subjectSrc Family Kinasesen_US
dc.subjectIl-10en_US
dc.subjectStat3en_US
dc.subjectLpsen_US
dc.titleLPS-induced Src family kinases activity mediates IL-10 production through activation of STAT3 in peripheral blood mononuclear cells of patients with Behcet's Diseaseen_US
dc.typeArticleen_US

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