Expression pattern and cellular localization of two critical non-nuclear progesterone receptors in the ovine corpus luteum during the estrous cycle and early pregnancy

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dc.contributor.authorAtli, Mehmet O.
dc.contributor.authorAkbalik, Mehmet Erdem
dc.contributor.authorKose, Mehmet
dc.contributor.authorAlak, Ilyas
dc.contributor.authorAtli, Zehra
dc.contributor.authorHitit, Mustafa
dc.date.accessioned2024-04-24T16:10:49Z
dc.date.available2024-04-24T16:10:49Z
dc.date.issued2022
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe study aimed to investigate the expression and cellular localization of two critical non-nuclear progesterone receptors, including membrane-associated-progesterone-receptor-component-1 (PGRMC1) and progestin and adipoQ receptor family member 7 (PAQR7) throughout the estrous cycle and early pregnancy in ovine corpus luteum (CL). Ewes were randomly grouped into cyclic (C, n = 4 per group) or pregnant (P, n = 4 per group) groups. Following slaughtering, the CL was obtained from both cyclic and pregnant ewes on days 12 (C12 and P12), 16 (C16 and P16), and 22 (C22 and P22). Western blotting and RT-qPCR were utilized to assess the expression levels of PGRMC1 and PAQR7, whereas immunohistochemistry was performed to determine the localization of PGRMC1 and PAQR7 in CL. Data were evaluated by one-way ANOVA, and the P < 0.05 was considered a significant difference. PGRMC1 was shown to be expressed in both small and large luteal cells and endothelial cells in CL, while PAQR7 expression was only found in small and large luteal cells. Compared to cycle days, pregnancy increased the expression of PGRMC1. PAQR7 did not differ during early pregnancy but reduced during the functional luteolysis stage (C16). mRNA and protein expression patterns for PGRMC1 and PAQR7 were similar on the studied days. This is the first study that demonstrates the expression and cellular localization of PGRMC1 and PAQR7 in ovine CL. We suggest that these receptors could execute a significant role in the ovine CL life span in both cyclic changes and the establishment of pregnancy.en_US
dc.description.sponsorship(College of Veterinary Medicine, at Colorado State University) [114O643]; DUBAP Project [VETERINER.19.006]; TUBITAK Project; DUBAP Project [114O643]; TUBITAK Project [VETERINER.19.006]en_US
dc.description.sponsorshipThe authors would like to express their special thanks to Drs. Aydin Guzeloglu and Ahmed Gad (the College of Veterinary Medicine, at Colorado State University) and Camden Oglesby (Department of Plant and Soil Sciences) for critically reviewing and editing the language of the manuscript. This study was supported by the DUBAP Project (VETERINER.19.006) and TUBITAK Project (114O643 to MK).en_US
dc.identifier.doi10.1016/j.anireprosci.2022.107026
dc.identifier.issn0378-4320
dc.identifier.issn1873-2232
dc.identifier.pmid35752032
dc.identifier.scopus2-s2.0-85132520554
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.anireprosci.2022.107026
dc.identifier.urihttps://hdl.handle.net/11468/15128
dc.identifier.volume243en_US
dc.identifier.wosWOS:000818512400007
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofAnimal Reproduction Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectProgesterone Membrane Receptorsen_US
dc.subjectSheepen_US
dc.subjectCorpus Luteumen_US
dc.titleExpression pattern and cellular localization of two critical non-nuclear progesterone receptors in the ovine corpus luteum during the estrous cycle and early pregnancyen_US
dc.titleExpression pattern and cellular localization of two critical non-nuclear progesterone receptors in the ovine corpus luteum during the estrous cycle and early pregnancy
dc.typeArticleen_US

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