A New UPLC Approach for the Simultaneous Quantitative Estimation of Four Compounds in a Cough Syrup Formulation

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dc.contributor.authorTurak, Fatma
dc.contributor.authorGuzel, Remziye
dc.contributor.authorDinc, Erdal
dc.date.accessioned2024-04-24T17:08:04Z
dc.date.available2024-04-24T17:08:04Z
dc.date.issued2016
dc.departmentDicle Üniversitesien_US
dc.description.abstractA new ultra-performance liquid chromatographic (UPLC) method was developed for the simultaneous estimation of potassium guaiacolsulfonate (PGS), guaifenesin (GUA), diphenhydramine HCl (DIP) and carbepentane citrate (CAR) in a commercial cough syrup. The chromatographic separation of four compounds PGS, GUA, DIP and CAR was performed on a BEH phenyl column (100 x 2.1 mm, 1.7 mu m i.d.) using a mobile phase consisting of acetonitrile and 0.1 MHCl (50 : 50, v/v). In addition, the optimized conditions of the chromatographic analysis were found with the flow rate of 0.38 mL/min, the column temperature of 30 degrees C and the injection volume of 1.2 mu L with the photodiode array detection of 220 nm. Calibration curves in the concentration ranges of 10-98 mu g/mL for PGS, 5-80 mu g/mL for GUA, 5-25 mu g/mL for DIP and CAR were computed by the regression of the analyte concentration on the chromatographic peak area. The newly developed UPLC method was validated by analyzing the quaternary mixtures of the related compounds, intraday and interday experiment and standard addition samples. After method validation, the proposed UPLC approach was successfully applied for the analysis of the commercial syrup formulation containing PGS, GUA, DIP and CAR compounds.en_US
dc.description.sponsorshipAnkara University [10A3336001]en_US
dc.description.sponsorshipThis study was performed at the Laboratory of Faculty of Pharmacy, which was supported by the scientific research fund of Ankara University (Project Number 10A3336001).en_US
dc.identifier.doi10.1093/chromsci/bmv102
dc.identifier.endpage35en_US
dc.identifier.issn0021-9665
dc.identifier.issn1945-239X
dc.identifier.issue1en_US
dc.identifier.pmid26202585
dc.identifier.scopus2-s2.0-84959918866
dc.identifier.scopusqualityQ3
dc.identifier.startpage28en_US
dc.identifier.urihttps://doi.org/10.1093/chromsci/bmv102
dc.identifier.urihttps://hdl.handle.net/11468/17185
dc.identifier.volume54en_US
dc.identifier.wosWOS:000372177400005
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherOxford Univ Press Incen_US
dc.relation.ispartofJournal of Chromatographic Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject[No Keyword]en_US
dc.titleA New UPLC Approach for the Simultaneous Quantitative Estimation of Four Compounds in a Cough Syrup Formulationen_US
dc.titleA New UPLC Approach for the Simultaneous Quantitative Estimation of Four Compounds in a Cough Syrup Formulation
dc.typeArticleen_US

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