Relative abundance and localization of interferon-stimulated gene 15 mRNA transcript in intra- and extra-uterine tissues during the early stages of pregnancy in sheep

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dc.contributor.authorAlak, Ilyas
dc.contributor.authorHitit, Mustafa
dc.contributor.authorKose, Mehmet
dc.contributor.authorKaya, M. Salih
dc.contributor.authorUcar, Eyyup Hakan
dc.contributor.authorAtli, Zehra
dc.contributor.authorAtli, Mehmet O.
dc.date.accessioned2024-04-24T16:10:49Z
dc.date.available2024-04-24T16:10:49Z
dc.date.issued2020
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe aim of this study was to investigate relative abundance and localization of ISG15 mRNA transcript in intra-uterine (trophoblast, endometrium) and extra-uterine (hypothalamus, anterior pituitary, corpus luteum) tissues before and during the period of conceptus implantation. Multiparous ewes (n = 16) were randomly allotted into four groups: pregnant or estrous cyclic on days of 12 and 16 (n = 4 per group) following estrus. Relative abundances of ISG15 mRNA transcript were determined in the endometrium, corpus luteum, hypothalamus, and anteriorpituitary using real time quantitative PCR. Localization of ISG15 mRNA transcript was evaluated using in situ hybridization. The presence of ISG15 mRNA transcript was only visualized in intrauterine tissues including the endometrium and trophoblast on day 12 of pregnancy. The ISG15 mRNA transcript was detected in all tissues evaluated on day 16 of pregnancy. The abundance of ISG15 mRNA transcript was greater in the endometrium on day 12 of pregnancy than at other days when evaluations occurred while in all other tissues except the hypothalamus there were large abundances of ISG15 mRNA on day 16 of pregnancy. It is concluded that the ISG15 mRNA transcript is only present in intra-uterine tissues before conceptus implantation. The ISG15 mRNA transcript, however, is present in extra-uterine tissues of ewes during implantation probably due to an increased amount of interferon-tau in blood circulation that is produced by the developing embryo. Results also indicate, for the first time, that pregnancy is associated with an intra-hypothalamus and anterior pituitary increased abundance of ISG15 mRNA transcript in ewes.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [214O643, 14-VF-112]en_US
dc.description.sponsorshipThe authors express their special thanks to Caitlin Hidalgo, Department of Animal and Dairy Science, Mississippi State University, Mississippi, USA for critical reviewing and language editing. This study was supported by a the Scientific and Technological Research Council of Turkey (TUBITAK) projects (214O643, and 14-VF-112 to MK, MOA)en_US
dc.identifier.doi10.1016/j.anireprosci.2020.106347
dc.identifier.issn0378-4320
dc.identifier.issn1873-2232
dc.identifier.pmid32414457
dc.identifier.scopus2-s2.0-85083398463
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.anireprosci.2020.106347
dc.identifier.urihttps://hdl.handle.net/11468/15126
dc.identifier.volume216en_US
dc.identifier.wosWOS:000532780600015
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofAnimal Reproduction Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectIsg15 Mrnaen_US
dc.subjectHypothalamusen_US
dc.subjectAnterior-Pituitaryen_US
dc.subjectPregnancyen_US
dc.subjectEwesen_US
dc.titleRelative abundance and localization of interferon-stimulated gene 15 mRNA transcript in intra- and extra-uterine tissues during the early stages of pregnancy in sheepen_US
dc.titleRelative abundance and localization of interferon-stimulated gene 15 mRNA transcript in intra- and extra-uterine tissues during the early stages of pregnancy in sheep
dc.typeArticleen_US

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