Evaluation of synergic effects of iodixanol and trehalose on cryosurvival of electroejaculated ram semen

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dc.contributor.authorOzmen, Mehmet Ferit
dc.contributor.authorCirit, Umut
dc.contributor.authorArici, Ramazan
dc.contributor.authorDemir, Kamber
dc.contributor.authorKurt, Dogan
dc.contributor.authorPabuccuoglu, Serhat
dc.contributor.authorAk, Kemal
dc.date.accessioned2024-04-24T17:11:25Z
dc.date.available2024-04-24T17:11:25Z
dc.date.issued2020
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe primary aim of the study was to investigate whether iodixanol and trehalose would have a synergic effect on the cryosurvival of electroejaculated ram semen. Tris-based diluter was used to prepare 9 different extenders by the addition of iodixanol or trehalose alone or varying combinations of these substances. Diluters were prepared as follows: Tris (control), Io5 (5% iodixanol), Tr25 (25 mmol/L trehalose), Tr50 (50 mmol/L trehalose), Tr50 + Io1.25 (50 mmol/L trehalose and 1.25% iodixanol), Tr50 + Io2.5 (50 mmol/L trehalose and 2.5% iodixanol), Tr50 + Io5 (50 mmol/L trehalose and 5% iodixanol), Tr25 + Io5 (25 mmol/L trehalose and 5% iodixanol) and Tr12.5 + Io5 (12.5 mmol/L trehalose and 5% iodixanol). Supplementation of the freezing extender with trehalose or iodixanol alone supported the protection of both morphological and functional integrity of ram spermatozoa and total motility at 1 and 4 hr post-thawing respectively. However, beyond these positive effects, the combination of trehalose (25 mmol/L) and iodixanol (5%) significantly increased post-thaw sperm longevity and motion properties at the end of 4-hr incubation. The results of the study clearly showed that there was positive synergic effect of iodixanol and trehalose on cryosurvival of ram semen.en_US
dc.identifier.doi10.1111/and.13656
dc.identifier.issn0303-4569
dc.identifier.issn1439-0272
dc.identifier.issue9en_US
dc.identifier.pmid32478874
dc.identifier.scopus2-s2.0-85085695506
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1111/and.13656
dc.identifier.urihttps://hdl.handle.net/11468/17496
dc.identifier.volume52en_US
dc.identifier.wosWOS:000536680200001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofAndrologia
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectElectroejaculated Semenen_US
dc.subjectFreezingen_US
dc.subjectIodixanolen_US
dc.subjectRamen_US
dc.subjectTrehaloseen_US
dc.titleEvaluation of synergic effects of iodixanol and trehalose on cryosurvival of electroejaculated ram semenen_US
dc.titleEvaluation of synergic effects of iodixanol and trehalose on cryosurvival of electroejaculated ram semen
dc.typeArticleen_US

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