Using cell banks as a tool in conservation programmes of native domestic breeds: the production of the first cloned Anatolian Grey cattle

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dc.contributor.authorArat, Sezen
dc.contributor.authorCaputcu, Arzu T.
dc.contributor.authorAkkoc, Tolga
dc.contributor.authorPabuccuoglu, Serhat
dc.contributor.authorSagirkaya, Hakan
dc.contributor.authorCirit, Umut
dc.contributor.authorNak, Yavuz
dc.date.accessioned2024-04-24T16:24:13Z
dc.date.available2024-04-24T16:24:13Z
dc.date.issued2011
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe aim of this study was to clone native Anatolian Grey cattle by using different donor cell types, such as fibroblast, cartilage and granulosa cells cryopreserved in a gene bank and oocytes aspirated from ovaries of Holstein cows as the recipient cytoplasm source. One male calf from fibroblast, three female calves from granulosa cells and one female calf from cartilage cells were born healthy and at normal birthweights. No calves were lost after birth. The results demonstrated that the cloned calves had the same microsatellite alleles at 11 loci as their nuclear donors. However, the mtDNAs of the five Anatolian Grey cloned calves had different haplotypes from their donor cells and mtDNA heteroplasmy could not be detected in any of the clones. The birth of healthy clones suggests that the haplotype difference between the cell and oocyte donor did not affect the pre- or post-implantation development of the bovine nuclear transfer derived embryos in our study. The results showed that well established nuclear transfer protocols could be useful in conserving endangered species. In conclusion, somatic cell banking can be suggested as a tool in conservation programmes of animal genetic resources.en_US
dc.description.sponsorshipIstanbul University; TUBITAK [TOVAG-104O360, KAMAG-106G005]en_US
dc.description.sponsorshipWe would like to thank Steven Stice, Yvan Heyman and Tayfur Tacirlioglu and Yuksel Agca for manuscript review; David Faber and David Wells for their advice about calf deliveries and neonatal care; Fatih Karakaya, Sakir Sekmen, Erman Ates, Gazi Turgut and Cihan Taskin for technical assistance; Kemal Ak, the Vice Dean of Istanbul University, for his support and cooperation; Sema Birler for moral support; Alev Kaymaz, Kursat Ozer, Zihni Mutlu, Gul Bakirer, Izem Hamzaoglu, Elif Karaman, Kaan Donmez, Gozde Ozalp, Gozde Simsek, Burcu Ustuner, Selin Alcay, Melih Erturk and Mustafa Uluoz for their help in recipient management, calf maintenance, calf deliveries and neonatal care. This research was supported by TUBITAK with grant numbers TOVAG-104O360 and KAMAG-106G005.en_US
dc.identifier.doi10.1071/RD11026
dc.identifier.endpage1023en_US
dc.identifier.issn1031-3613
dc.identifier.issue8en_US
dc.identifier.pmid22127006
dc.identifier.scopus2-s2.0-80054123511
dc.identifier.scopusqualityQ2
dc.identifier.startpage1012en_US
dc.identifier.urihttps://doi.org/10.1071/RD11026
dc.identifier.urihttps://hdl.handle.net/11468/16590
dc.identifier.volume23en_US
dc.identifier.wosWOS:000295838700008
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherCsiro Publishingen_US
dc.relation.ispartofReproduction Fertility and Development
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCloningen_US
dc.subjectCryopreservationen_US
dc.subjectHaplotypesen_US
dc.subjectMtdnaen_US
dc.subjectNuclear Transferen_US
dc.subjectTelomereen_US
dc.titleUsing cell banks as a tool in conservation programmes of native domestic breeds: the production of the first cloned Anatolian Grey cattleen_US
dc.titleUsing cell banks as a tool in conservation programmes of native domestic breeds: the production of the first cloned Anatolian Grey cattle
dc.typeArticleen_US

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