Production and characterization of neutral and alkaline protease from different Bacillus subtilis strains

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dc.contributor.authorUyar, Fikret
dc.contributor.authorBaysal, Zuebeyde
dc.date.accessioned2024-04-24T17:37:50Z
dc.date.available2024-04-24T17:37:50Z
dc.date.issued2008
dc.departmentDicle Üniversitesien_US
dc.description.abstractAn extracellular neutral and alkaline protease from two different Bacillus subtilis strains were studied. The optimal activity occured when the pH level was 7.0 and 10.5 at a temperature of 35 and 45 degrees C for neutral protease and alkaline protease, respectively. When neutral protease was stable in the temperature range 35-60 degrees C, alkaline protease was found stable between 35-55 degrees C for 0.5 h. Divalent cations, especially Ca2+ increased enzymes activity and were inhibited by Mn2+,Ni2+, CU2+, Fe2+, Co2+, Cd2+ and Hg2+ for neutral protease and by Zn2+, Cd2+,Co2+, Cu2+ and Hg2+ for alkaline protease. The neutral protease was also inhibited by ethylenediamine-tetraacetic acid, 1, 10 phenanthroline and dithiothreitol whereas alkaline protease was inhibited with phenylmethyl-sulfonyl fluoride. The obtained K-m values were 2.28 x 10(-3) +/- 3.65 x 10(-4), 2.28 x 10(-4) +/- 4.21 x 10(-5) and 1.70 x 10(-4) +/- 5.18 x 10(-5) M for azocasein, BSA and casein for neutral protease, respectively. The K-m values with azocasein, BSA, casein, N-suc-Ala-Ala-Ala-pNA and N-cbz-Ala-Ala-Leu-pNA were 2.02 x 10(-3) +/- 7.3 x 10(-5), 2.13 x 10(-4) +/- 6.04 x 10(-5), 8.75 x 10(-4) +/- 1.36 x 10(-4), 1.71 x 10(-3) +/- 3.93 x 10(-4) and 2.64 x 10(-4) +/- 4.93 x 10(-5) for alkaline protease.en_US
dc.identifier.endpage4530en_US
dc.identifier.issn0970-7077
dc.identifier.issn0975-427X
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-54049104044
dc.identifier.scopusqualityQ4
dc.identifier.startpage4523en_US
dc.identifier.urihttps://hdl.handle.net/11468/21204
dc.identifier.volume20en_US
dc.identifier.wosWOS:000259602700059
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherAsian Journal Of Chemistryen_US
dc.relation.ispartofAsian Journal of Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBacillus Subtilisen_US
dc.subjectNeutral Proteaseen_US
dc.subjectAlkaline Proteaseen_US
dc.subjectPropertiesen_US
dc.titleProduction and characterization of neutral and alkaline protease from different Bacillus subtilis strainsen_US
dc.titleProduction and characterization of neutral and alkaline protease from different Bacillus subtilis strains
dc.typeArticleen_US

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