Production and characterization of neutral and alkaline protease from different Bacillus subtilis strains
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Tarih
2008
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Asian Journal Of Chemistry
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
An extracellular neutral and alkaline protease from two different Bacillus subtilis strains were studied. The optimal activity occured when the pH level was 7.0 and 10.5 at a temperature of 35 and 45 degrees C for neutral protease and alkaline protease, respectively. When neutral protease was stable in the temperature range 35-60 degrees C, alkaline protease was found stable between 35-55 degrees C for 0.5 h. Divalent cations, especially Ca2+ increased enzymes activity and were inhibited by Mn2+,Ni2+, CU2+, Fe2+, Co2+, Cd2+ and Hg2+ for neutral protease and by Zn2+, Cd2+,Co2+, Cu2+ and Hg2+ for alkaline protease. The neutral protease was also inhibited by ethylenediamine-tetraacetic acid, 1, 10 phenanthroline and dithiothreitol whereas alkaline protease was inhibited with phenylmethyl-sulfonyl fluoride. The obtained K-m values were 2.28 x 10(-3) +/- 3.65 x 10(-4), 2.28 x 10(-4) +/- 4.21 x 10(-5) and 1.70 x 10(-4) +/- 5.18 x 10(-5) M for azocasein, BSA and casein for neutral protease, respectively. The K-m values with azocasein, BSA, casein, N-suc-Ala-Ala-Ala-pNA and N-cbz-Ala-Ala-Leu-pNA were 2.02 x 10(-3) +/- 7.3 x 10(-5), 2.13 x 10(-4) +/- 6.04 x 10(-5), 8.75 x 10(-4) +/- 1.36 x 10(-4), 1.71 x 10(-3) +/- 3.93 x 10(-4) and 2.64 x 10(-4) +/- 4.93 x 10(-5) for alkaline protease.
Açıklama
Anahtar Kelimeler
Bacillus Subtilis, Neutral Protease, Alkaline Protease, Properties
Kaynak
Asian Journal of Chemistry
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
20
Sayı
6
