The evaluation of antioxidant and anticancer effects of Lepidium Sativum Subsp Spinescens L. methanol extract on cancer cells

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dc.contributor.authorSelek, Sahabettin
dc.contributor.authorKoyuncu, Ismail
dc.contributor.authorCaglar, Hifa Gulru
dc.contributor.authorBektas, Ibrahim
dc.contributor.authorYilmaz, Mustafa Abdullah
dc.contributor.authorGonel, Ataman
dc.contributor.authorAkyuz, Enes
dc.date.accessioned2024-04-24T17:17:57Z
dc.date.available2024-04-24T17:17:57Z
dc.date.issued2018
dc.departmentDicle Üniversitesien_US
dc.description.abstractIn recent years, there is an increased research interest for plants which are natural sources of antioxidants. Lepidium sativum Subsp spinescens L., commonly found in South West Asia, is a plant known as a healthy nutritional source containing bio-molecules that carry anti-hypertensive, hypoglycemic, antiasthmatic, antispasmodic, hepato-protective, chemoprotective, anti-inflammatory and anti-oxidant effects. In this study, we aimed to investigate the antioxidant content and activity of Lepidium sativum Subsp spinescens L. methanol extract on cancer cells. Methanol extract of dried Lepidium sativum Subsp spinescens L. was prepared. Total amount of phenolic compounds was determined by Slinkard and Singleton method using Folin-Ciocalteu reagent. Total flavonoid amount was determined according to Zhishen method. Antioxidant activity of the extract was evaluated by CUPRAC and ABTS radical scavenging activity assays. Cytotoxic effects of the plant extract on colon and endometrium cancer cells, and human peripheral lymphocyte cells were investigated in vitro by MTT and neutral red assays. Furthermore, the plant extract was investigated for necrotic effects by LDH assay; apoptotic activity by DNA ladder fragmentation, ELISA and acridine orange/ethidium bromide staining; and genotoxic effect by comet assay methods. Methanol extract of Lepidium sativum Subsp spinescens L. was found to have a high content of phenolic and flavonoid compounds. The extract showed significant antioxidant activity and also cytotoxic activity on colon and endometrium cancer cells in a concentration-dependent manner. Apoptotic activity and genotoxic effects were significantly increased, especially with 200 mu g/ml concentrations at 48 hours incubation. In conclusion, it was determined that the extract evaluated in this study could be a natural source of antioxidants. Further molecular studies explaining chemo-preventive and chemotherapeutic effects on cancer cells are required to support anticancer efficacy of the plant.en_US
dc.identifier.doi10.14715/cmb/2018.64.3.12
dc.identifier.endpage80en_US
dc.identifier.issn0145-5680
dc.identifier.issn1165-158X
dc.identifier.issue3en_US
dc.identifier.pmid29506633
dc.identifier.scopus2-s2.0-85042752068
dc.identifier.scopusqualityQ4
dc.identifier.startpage72en_US
dc.identifier.urihttps://doi.org/10.14715/cmb/2018.64.3.12
dc.identifier.urihttps://hdl.handle.net/11468/18474
dc.identifier.volume64en_US
dc.identifier.wosWOS:000426541100012
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherC M B Assocen_US
dc.relation.ispartofCellular and Molecular Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLepidium Sativum Subsp Spinescens L.en_US
dc.subjectAntioxidant Activityen_US
dc.subjectAnticancer Activityen_US
dc.titleThe evaluation of antioxidant and anticancer effects of Lepidium Sativum Subsp Spinescens L. methanol extract on cancer cellsen_US
dc.titleThe evaluation of antioxidant and anticancer effects of Lepidium Sativum Subsp Spinescens L. methanol extract on cancer cells
dc.typeArticleen_US

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