Optimizing pectin lyase production using the one-factor-at-a-time method and response surface methodology

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dc.authoridGUL, Ertugrul/0000-0001-8611-2983
dc.authoridTepe, Ozlem/0000-0001-8336-3699
dc.contributor.authorGul, Ertugrul
dc.contributor.authorDursun, Arzu Yadigar
dc.contributor.authorTepe, Ozlem
dc.contributor.authorAkaslan, Gonca
dc.contributor.authorPampal, Fadile Gul
dc.date.accessioned2025-02-22T14:09:11Z
dc.date.available2025-02-22T14:09:11Z
dc.date.issued2024
dc.departmentDicle Üniversitesien_US
dc.description.abstractPectinases are commonly industrially synthesized by molds. This study aimed to optimize pectin lyase synthesis by a bacterium, Pseudomonas fluorescens, using both the one-factor-at-a-time (OFAT) method and response surface methodology. First, on optimization of pectin lyase fermentation by the OFAT method, the effects of pectin, peptone, yeast extract, (NH4)(2)SO4, pH, and salts were investigated. The highest pectin lyase activity was determined to be 28.63 U/mL at pH 8, 30 degrees C, with 1% (w/v) pectin and 0.14% (w/v) (NH4)(2)SO4 concentration at the 90th hour. The effect of substrate inhibition on the microbial growth was also investigated, and the results showed that the process can be described by noncompetitive inhibition model. The values of kinetic constants were determined as mu (m) = 0.175 h(-1), K-S = 6.931 g/L, and, K-I = 6.932 g/L by nonlinear regression analysis. It was reported that pectin lyase enzymes exhibited peak activity at 50 degrees C and pH 8. Finally, response surface methodology (RSM) was utilized to optimize pH, concentrations of ammonium sulfate, and pectin, which were chosen as independent variables. The interactions between these variables were also examined. According to RSM, the optimum values of the parameters to achieve a maximum pectin lyase activity of 35.62 U/mL were determined to be pH 7.97, 1.25% (w/v) pectin concentration, and 0.25% (w/v) (NH4)(2)SO4 concentration.en_US
dc.description.sponsorshipFirat University Scientific Research Projects Unit (FUBAP), Turkey [FUBAP MF. 12.11]en_US
dc.description.sponsorshipFirat University Scientific Research Projects Unit (FUBAP), Turkey,Grant/Award Number: FUBAP MF. 12.11en_US
dc.identifier.doi10.1002/bab.2686
dc.identifier.issn0885-4513
dc.identifier.issn1470-8744
dc.identifier.pmid39434440en_US
dc.identifier.scopus2-s2.0-85206942843en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1002/bab.2686
dc.identifier.urihttps://hdl.handle.net/11468/29828
dc.identifier.wosWOS:001339684300001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofBiotechnology and Applied Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKA_WOS_20250222
dc.subjectenzyme stabilityen_US
dc.subjectmicrobial kineticen_US
dc.subjectoptimizationen_US
dc.subjectpectin lyaseen_US
dc.subjectPseudomonas fluorescensen_US
dc.subjectresponse surface methodologyen_US
dc.titleOptimizing pectin lyase production using the one-factor-at-a-time method and response surface methodologyen_US
dc.typeArticleen_US

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