Synthesis of a mixed-model stationary phase derived from glutamine for HPLC separation of structurally different biologically active compounds: HILIC and reversed-phase applications

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dc.contributor.authorAral, Tarik
dc.contributor.authorAral, Hayriye
dc.contributor.authorZiyadanogullari, Berrin
dc.contributor.authorZiyadanogullari, Recep
dc.date.accessioned2024-04-24T16:18:25Z
dc.date.available2024-04-24T16:18:25Z
dc.date.issued2015
dc.departmentDicle Üniversitesien_US
dc.description.abstractA novel mixed-mode stationary phase was synthesised starting from N-Boc-glutamine, aniline and spherical silica gel (4 mu m, 60 angstrom). The prepared stationary phase was characterized by IR and elemental analysis. The new stationary phase bears an embedded amide group into phenyl ring, highly polar a terminal amide group and non-polar groups (phenyl and alkyl groups). At first, this new mixed-mode stationary phase was used for HILIC separation of four nucleotides and five nucleosides. The effects of different separation conditions, such as pH value, mobile phase and temperature, on the separation process were investigated. The optimum separation for nucleotides was achieved using HILIC isocratic elution with aqueous mobile phase and acetonitrile with 20 degrees C column temperature. Under these conditions, the four nucleotides could be separated and detected at 265 nm within 14 min. Five nucleosides were separated under HILIC isocratic elution with aqueous mobile phase containing pH=3.25 phosphate buffer (10 mM) and acetonitrile with 20 degrees C column temperature and detected at 265 nm within 14 min. Chromatographic parameters as retention factor, selectivity, theoretical plate number and peak asymmetry factor were calculated for the effect of temperature and water content in mobile phase on the separation process. The new column was also tested for nucleotides and nucleosides mixture and six analytes were separated in 10 min. The chromatographic behaviours of these polar analytes on the new mixed-model stationary phase were compared with those of HILIC columns under similar conditions. Further, phytohormones and phenolic compounds were separated in order to see influence of the new stationary phase in reverse phase conditions. Eleven plant phytohormones were separated within 13 min using RP-HPLC gradient elution with aqueous mobile phase containing pH=2.5 phosphate buffer (10 mM) and acetonitrile with 20 degrees C column temperature and detected at 230 or 278 nm. The best separation conditions for seven phenolic compounds was also achieved using reversed-phase HPLC gradient elution with aqueous mobile phase containing pH=2.5 phosphate buffer (10 mM) and acetonitrile with 20 degrees C column temperature and seven phenolic compounds could be separated and detected at 230 nm within 16 min. (C) 2014 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.talanta.2014.07.060
dc.identifier.endpage73en_US
dc.identifier.issn0039-9140
dc.identifier.issn1873-3573
dc.identifier.pmid25281074
dc.identifier.scopus2-s2.0-84906328217
dc.identifier.scopusqualityQ1
dc.identifier.startpage64en_US
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2014.07.060
dc.identifier.urihttps://hdl.handle.net/11468/16081
dc.identifier.volume131en_US
dc.identifier.wosWOS:000343691000010
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofTalanta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMixed-Model Stationary Phaseen_US
dc.subjectPolar Stationary Phaseen_US
dc.subjectNucleotides And Nucleosidesen_US
dc.subjectPhytohormonesen_US
dc.subjectPhenolic Compoundsen_US
dc.subjectHilicen_US
dc.titleSynthesis of a mixed-model stationary phase derived from glutamine for HPLC separation of structurally different biologically active compounds: HILIC and reversed-phase applicationsen_US
dc.titleSynthesis of a mixed-model stationary phase derived from glutamine for HPLC separation of structurally different biologically active compounds: HILIC and reversed-phase applications
dc.typeArticleen_US

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