Diagnosis of q fever and brucellosis in aborted ovine fetuses by microbiological, pathological and immunohistochemical methods

dc.authorid0000-0002-2952-5180en_US
dc.authorid0000-0002-5456-697Xen_US
dc.contributor.authorYeşilmen, Simten
dc.contributor.authorYaman, Turan
dc.contributor.authorSağsöz, Hakan
dc.contributor.authorBademkıran, Servet
dc.date.accessioned2022-12-12T06:32:30Z
dc.date.available2022-12-12T06:32:30Z
dc.date.issued2018en_US
dc.departmentDicle Üniversitesi, Veteriner Fakültesi, Klinik Öncesi Bilimler Bölümü, Mikrobiyoloji Ana Bilim Dalıen_US
dc.description.abstractBrucellosis and Q fever, two zoonoses, are important causes of abortion in ruminants, as well as economically significant diseases caused by a gram-negative bacterium. Determination of these diseases is therefore of great importance. In this study, the organs of 35 naturally infected and aborted ovine fetuses were examined for the presence of changes resulting from infections by Brucella melitensis and Coxiella burnetii, according to macroscopic, bacteriological, histopathological and immunohistochemical methods. B. melitensis was observed in 21 cases, and C. burnetii was observed in 8 cases of the aborted ovine fetuses, and these were determined with immunohistochemical methods. Brucellosis was observed in 18 of the aborted ovine fetuses, and this was determined by microbiological methods. Negative (-) results were found for all of the other fetuses. The Brucella antigen was determined to be localized as intracytoplasmic in mainly alveolar macrophages, bronchi, bronchioles, glandular epithelial cells around bronchial glands, neutrophils, hepatocytes and Kupffer cells. The Coxiella antigen was found to be localized in the alveolar macrophages in the lungs, bronchi, bronchioles and alveolus, and in the cytoplasms of bronchial gland epithelial cells, and in the cytoplasms of hepatocytes and Kupffer cells in the liver. Immunohistochemical and microbiological diagnoses of brucellosis and coxiellosis were compared; it was concluded that immunohistochemical methods were more safely applied than microbiological methods.en_US
dc.identifier.citationYeşilmen, S., Yaman, T., Sağsöz, H. ve Bademkıran, S. (2018). Diagnosis of q fever and brucellosis in aborted ovine fetuses by microbiological, pathological and immunohistochemical methods. Acta Veterinaria, 68(2), 168-177.en_US
dc.identifier.doi10.2478/acve-2018-0013
dc.identifier.endpage177en_US
dc.identifier.issn0567-8315
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85050272684
dc.identifier.scopusqualityQ3
dc.identifier.startpage168en_US
dc.identifier.urihttps://sciendo.com/article/10.2478/acve-2018-0013
dc.identifier.urihttps://hdl.handle.net/11468/11015
dc.identifier.volume68en_US
dc.identifier.wosWOS:000438055500003
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorYeşilmen, Simten
dc.institutionauthorSağsöz, Hakan
dc.institutionauthorBademkıran, Servet
dc.language.isoenen_US
dc.publisherWalter de Gruyter GmbHen_US
dc.relation.ispartofActa Veterinaria
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAbortusen_US
dc.subjectBrucella melitensisen_US
dc.subjectImmunohistochemistryen_US
dc.subjectQ feveren_US
dc.titleDiagnosis of q fever and brucellosis in aborted ovine fetuses by microbiological, pathological and immunohistochemical methodsen_US
dc.titleDiagnosis of q fever and brucellosis in aborted ovine fetuses by microbiological, pathological and immunohistochemical methods
dc.typeArticleen_US

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