Purification and properties of glucose 6-phosphate dehydrogenase from turkey erythrocytes

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dc.contributor.authorYilmaz, H
dc.contributor.authorÇiftçi, M
dc.contributor.authorBeydemir, S
dc.contributor.authorBakan, E
dc.contributor.authorKüfrevioglu, ÖI
dc.date.accessioned2024-04-24T17:37:53Z
dc.date.available2024-04-24T17:37:53Z
dc.date.issued2003
dc.departmentDicle Üniversitesien_US
dc.description.abstractGlucose 6-phosphate dehydrogenase (G6PD) was purified from turkey erythrocytes by ammonium sulphate precipitation and followed by ADP Sepharose affinity gel chromatography. The yield was 49.71% and specific activity of the enzyme was found to be 4.4.16 EU/mg protein. By gel filtration the molecular mass was found to be 75 kDa. The enzyme had an optimum pH at 9.0, and opt;mum temperature at 50degreesC. K-m and V-max for NADP(+) and glucose 6- phosphate (G6-P) as substrates were also determined and effects of inhibitors such as ATP, NADH and NADPH were examined.en_US
dc.identifier.endpage65en_US
dc.identifier.issn0301-1208
dc.identifier.issn0975-0959
dc.identifier.issue1en_US
dc.identifier.pmid22900294
dc.identifier.scopus2-s2.0-0038201831
dc.identifier.scopusqualityQ3
dc.identifier.startpage62en_US
dc.identifier.urihttps://hdl.handle.net/11468/21228
dc.identifier.volume40en_US
dc.identifier.wosWOS:000181299000010
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherNatl Inst Science Communication-Niscairen_US
dc.relation.ispartofIndian Journal of Biochemistry & Biophysics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject[No Keyword]en_US
dc.titlePurification and properties of glucose 6-phosphate dehydrogenase from turkey erythrocytesen_US
dc.titlePurification and properties of glucose 6-phosphate dehydrogenase from turkey erythrocytes
dc.typeArticleen_US

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