Effects of melatonin on apoptosis and cell differentiation in MCF-7 derived cancer stem cells

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dc.contributor.authorKocak, Nadir
dc.contributor.authorDonmez, Huseyin
dc.contributor.authorYildirim, Ibrahim Halil
dc.date.accessioned2024-04-24T17:17:57Z
dc.date.available2024-04-24T17:17:57Z
dc.date.issued2018
dc.departmentDicle Üniversitesien_US
dc.description.abstractMelatonin is a hormone of the pineal gland that has a wide range of biological effects such as antioxidant, anti-inflammatory, and anti-tumor activity. Previous studies have shown that melatonin also affects survival, proliferation, and apoptosis of the cells. In this study, we investigated the effect of melatonin on apoptosis, self-renewal, and differentiation. For this purpose, MCF-7 and HEK293 cells were subjected to melatonin treatment. Expression of genes related to apoptosis (Bax and Bcl2) and self-renewal and differentiation (Oct4, Sox2, and Nanog) analyzed after the sorting of cancer stem cells from MCF-7 cells. Results showed that the effect of melatonin is dependent on the melatonin concentration and treatment periods. Melatonin treatment decreased the cell proliferation rate of MCF-7 in contrast to HEK293. Also, this treatment increased apoptosis in MCF-7 cells and decreased in HEK293 cells. Gene expression of Nanog was decreased and Sox2 was increased in both cell groups after the melatonin treatment. Expression of Oct4 was decreased in MCF-7 cells and increased in HEK293 cells. We determined that melatonin decreases apoptosis and differentiation of stem cells in normal HEK293 stem cells, but increases apoptosis and differentiation in the MCF-7 cancer stem cells.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [1002-113S478]en_US
dc.description.sponsorshipThis study was supported by a research grant from the Scientific and Technological Research Council of Turkey (TUBITAK, Grant No: 1002-113S478).en_US
dc.identifier.doi10.14715/cmb/2018.64.12.12
dc.identifier.endpage61en_US
dc.identifier.issn0145-5680
dc.identifier.issn1165-158X
dc.identifier.issue12en_US
dc.identifier.pmid30301504
dc.identifier.scopus2-s2.0-85054776618
dc.identifier.scopusqualityQ4
dc.identifier.startpage56en_US
dc.identifier.urihttps://doi.org/10.14715/cmb/2018.64.12.12
dc.identifier.urihttps://hdl.handle.net/11468/18472
dc.identifier.volume64en_US
dc.identifier.wosWOS:000449068700012
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherC M B Assocen_US
dc.relation.ispartofCellular and Molecular Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCancer Stem Cellsen_US
dc.subjectMelatoninen_US
dc.subjectApoptosisen_US
dc.subjectSelf-Renewalen_US
dc.titleEffects of melatonin on apoptosis and cell differentiation in MCF-7 derived cancer stem cellsen_US
dc.titleEffects of melatonin on apoptosis and cell differentiation in MCF-7 derived cancer stem cells
dc.typeArticleen_US

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