Detection and characterisation of the genes encoding glyoxalase I and II from Neisseria meningitidis

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dc.contributor.authorKızıl, Göksel
dc.contributor.authorWilks, Kathryn
dc.contributor.authorWells, Damien
dc.contributor.authorAla'Aldeen, Dlawer A.A.
dc.date.accessioned2024-04-24T17:56:24Z
dc.date.available2024-04-24T17:56:24Z
dc.date.issued2000
dc.departmentDicle Üniversitesien_US
dc.description.abstractGlyoxalase enzymes I and II are involved in a detoxification process consisting of conversion of reactive dicarbonyl compounds (e.g., methylglyoxal) to less reactive hydroxy acids. The structural gene for meningococcal glyoxalase I (gloA) was identified by screening an expression library with a rabbit antiserum. The meningococcal gloA gene consisted of 138 deduced amino acids, with a calculated mol. wt of 15.7 kDa. The DNA and deduced protein sequence of gloA was compared to known sequences of glyoxalase I enzymes and showed high homology with gloA of several eukaryotic and prokaryotic species. Insertion of a gloA-containing plasmid in Escherichia coli increased the host organism's tolerance to methylglyoxal from <2 mM to >4 mM, thus demonstrating its functional identity. A databank search also revealed the presence of a putative gloB gene, encoding glyoxalase II (GlxII), in the recently released genomic sequences of Neisseria meningitidis and N. gonorrhoeae.en_US
dc.identifier.citationKızıl, G., Wilks, K., Wells, D. ve Ala'Aldeen, D. A. A. (2000). Detection and characterisation of the genes encoding glyoxalase I and II from Neisseria meningitidis. Journal of Medical Microbiology, 49(7), 669-673.
dc.identifier.doi10.1099/0022-1317-49-7-669
dc.identifier.endpage673en_US
dc.identifier.issn0022-2615
dc.identifier.issue7en_US
dc.identifier.pmid10882093
dc.identifier.scopus2-s2.0-0033923358
dc.identifier.scopusqualityQ2
dc.identifier.startpage669en_US
dc.identifier.urihttps://doi.org/10.1099/0022-1317-49-7-669
dc.identifier.urihttps://hdl.handle.net/11468/23492
dc.identifier.urihttps://www.microbiologyresearch.org/content/journal/jmm/10.1099/0022-1317-49-7-669
dc.identifier.volume49en_US
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherLippincott Williams and Wilkinsen_US
dc.relation.ispartofJournal of Medical Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleDetection and characterisation of the genes encoding glyoxalase I and II from Neisseria meningitidisen_US
dc.titleDetection and characterisation of the genes encoding glyoxalase I and II from Neisseria meningitidis
dc.typeArticleen_US

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