Detection and characterisation of the genes encoding glyoxalase I and II from Neisseria meningitidis
Yükleniyor...
Tarih
2000
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Lippincott Williams and Wilkins
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Glyoxalase enzymes I and II are involved in a detoxification process consisting of conversion of reactive dicarbonyl compounds (e.g., methylglyoxal) to less reactive hydroxy acids. The structural gene for meningococcal glyoxalase I (gloA) was identified by screening an expression library with a rabbit antiserum. The meningococcal gloA gene consisted of 138 deduced amino acids, with a calculated mol. wt of 15.7 kDa. The DNA and deduced protein sequence of gloA was compared to known sequences of glyoxalase I enzymes and showed high homology with gloA of several eukaryotic and prokaryotic species. Insertion of a gloA-containing plasmid in Escherichia coli increased the host organism's tolerance to methylglyoxal from <2 mM to >4 mM, thus demonstrating its functional identity. A databank search also revealed the presence of a putative gloB gene, encoding glyoxalase II (GlxII), in the recently released genomic sequences of Neisseria meningitidis and N. gonorrhoeae.
Açıklama
Anahtar Kelimeler
Kaynak
Journal of Medical Microbiology
WoS Q Değeri
Scopus Q Değeri
Q2
Cilt
49
Sayı
7
Künye
Kızıl, G., Wilks, K., Wells, D. ve Ala'Aldeen, D. A. A. (2000). Detection and characterisation of the genes encoding glyoxalase I and II from Neisseria meningitidis. Journal of Medical Microbiology, 49(7), 669-673.
