Effect of transport and storage temperature of ovaries on in vitro maturation of bitch oocytes

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dc.contributor.authorTas, Muzaffer
dc.contributor.authorEvecen, Mithat
dc.contributor.authorOzdas, Ozen Banu
dc.contributor.authorCirit, Umut
dc.contributor.authorDemir, Kamber
dc.contributor.authorBirler, Sema
dc.contributor.authorPabuccuoglu, Serhat
dc.date.accessioned2024-04-24T16:10:47Z
dc.date.available2024-04-24T16:10:47Z
dc.date.issued2006
dc.departmentDicle Üniversitesien_US
dc.description.abstractIn this study, the effects of ovary transport and storage temperature on in vitro maturation of bitch oocytes were investigated. Ovaries were collected from 23 mature bitches and one randomly selected ovary of each pair (n = 23 pairs) was transported in physiologic saline at 4 degrees C, while the other one at 35-38 degrees C for 2-4 h. A total of 316 cumulus oocyte complexes (COCs) were obtained from the 4 degrees C group and 301 COCs from the 35-38 degrees C group. All COCs were matured in modified synthetic oviduct fluid (mSOF) supplemented with follicle stimulating hormone (FSH), essential and non-essential amino acids at 38 degrees C in ahumidified 5% CO2, 5% O-2, and 90% N-2 atmosphere for 72 h. At the end of the in vitro maturation period, nuclear maturation of oocytes was classified as germinal vesicle (GV), germinal vesicle breakdown (GVBD), metaphase I (MI), metaphase II (MII), undetermined nuclear maturation (UDNM), and MI + MII. The nuclear maturation rates to MI, MII, and MI + MII stages were 60.44%, 10.75%, and 71.20% in the 4 degrees C group and 37.20%, 7.64%, and 45.85% in the 35-38 degrees C group, respectively. The data demonstrated that oocytes obtained from ovaries transported at 4 degrees C had higher maturation rates than from the ones transported at 35-38 degrees C (p < 0.001). (c) 2005 Published by Elsevier B.V.en_US
dc.identifier.doi10.1016/j.anireprosci.2005.11.001
dc.identifier.endpage34en_US
dc.identifier.issn0378-4320
dc.identifier.issue1-2en_US
dc.identifier.pmid16343825
dc.identifier.scopus2-s2.0-33748461021
dc.identifier.scopusqualityQ1
dc.identifier.startpage30en_US
dc.identifier.urihttps://doi.org/10.1016/j.anireprosci.2005.11.001
dc.identifier.urihttps://hdl.handle.net/11468/15112
dc.identifier.volume96en_US
dc.identifier.wosWOS:000241148300004
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofAnimal Reproduction Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDogen_US
dc.subjectOvaryen_US
dc.subjectTemperatureen_US
dc.subjectOocyteen_US
dc.subjectIn Vitro Maturationen_US
dc.titleEffect of transport and storage temperature of ovaries on in vitro maturation of bitch oocytesen_US
dc.titleEffect of transport and storage temperature of ovaries on in vitro maturation of bitch oocytes
dc.typeArticleen_US

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